Effect of prior application with and without post-injury treatment with low-level laser on the modulation of key proteins in the muscle repair process

Autor(es)

Rodrigues, Danielle De Lima ^[1] ; Alves, Agnelo Neves ^[1] ; Guimaraes, Beatriz Ribeiro ^[2] ; de Alcantara Araujo Amorim, Weslley Wallace ^[3] ; Bussadori, Sandra Kalil ^{[2, 1]} ; Santos Fernandes, Kristianne Porta ^[1] ; Mesquita-Ferrari, Raquel Agnelli ^{[2, 1, 4]}

Afiliação do(s) autor(es)

^[1] Univ Nove Julho UNINOVE, Postgrad Program Biophoton Appl Hlth Sci, Sao Paulo, SP - Brazil

^[2] Univ Nove Julho UNINOVE, Postgrad Program Rehabil Sci, Sao Paulo, SP - Brazil

^[3] Univ Nove Julho UNINOVE, Sao Paulo, SP - Brazil

^[4] Univ Nove Julho UNINOVE, Dept Pos Grad, Ciencias Reabil, Rua Vergueiro 235-249, BR-01504001 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Lasers in Medical Science; v. 33, n. 6, p. 1207-1213, AUG 2018.
• Citações Web of Science: 0

Resumo

The aim of the present study was to evaluate the effects of LLLT prior to muscle injury with and without post-injury irradiation on the expression of isoforms of myosin heavy chain (MyHC), calcineurin (CaN), and myostatin during the repair process. Wistar rats were divided into five groups: control (n = 7); injury (n = 21); LLLT + injury (n = 21); injury + LLLT (n = 21), and LLLT + injury + LLLT (n = 21). Cryoinjury was performed on the tibialis anterior (TA) muscle. The injured groups were euthanized at 3, 7, and 14 days after injury. LLLT was performed using an infrared laser (780 nm) with the following parameters: 10 J/cm(2), 40 mW, 10 s per point, 8 points, and 3.2 J of total energy. At the end of each period, the TA muscle was removed for the analysis of MyHC, CaN, and myostatin gene expression using real-time PCR. The data were tested statistically by Kruskal-Wallis with Dunn's post hoc test (p < 0.05). The results demonstrated that prior irradiation reduced the mRNA expression of all proteins at 3 days. Post irradiation reduced the mRNA expression of MyHC-1, MyHC-2a, MyHC-2b, and CaN at 7 days. Prior irradiation combined with post-injury irradiation reduced the mRNA expression of MyHC-2x and CaN at 14 days and increased the mRNA expression of myostatin in the same period. In conclusion, different protocols of photobiomodulation can modulate the expression of the different isoforms of MyHC, CaN, and myostatin during the repair process. It is noteworthy that the combination of the prior and post-injury irradiation was the protocol that most promoted changes in the final phase of the repair process. (AU)

• Processo FAPESP: 17/06461-0 - Efeitos da laserterapia e do ultrassom terapêutico na expressão proteica de isoformas de cadeia pesada de miosina durante o reparo do músculo esquelético de ratos
• Beneficiário: Weslley Wallace de Alcântara Araujo Amorim
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 14/12381-1 - Análise comparativa do efeito da laserterapia prévia associada ou não ao tratamento pós sobre a modulação de citocinas inflamatórias, do estresse oxidativo e a determinação do tipo de fibra muscular após lesão aguda
• Beneficiário: Raquel Agnelli Mesquita Ferrari
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/21540-3 - Efeito comparativo da terapia laser de baixa potência e do ultrassom da baixa intensidade sobre o fenótipo de fibras musculares, estresse oxidativo e proteínas envolvidas na regulação do reparo muscular
• Beneficiário: Agnelo Neves Alves
• Modalidade de apoio: Bolsas no Brasil - Doutorado