Effect of prior application with and without post-injury treatment with low-level laser on the modulation of key proteins in the muscle repair process

Author(s)

Rodrigues, Danielle De Lima ^[1] ; Alves, Agnelo Neves ^[1] ; Guimaraes, Beatriz Ribeiro ^[2] ; de Alcantara Araujo Amorim, Weslley Wallace ^[3] ; Bussadori, Sandra Kalil ^{[2, 1]} ; Santos Fernandes, Kristianne Porta ^[1] ; Mesquita-Ferrari, Raquel Agnelli ^{[2, 1, 4]}

Affiliation

^[1] Univ Nove Julho UNINOVE, Postgrad Program Biophoton Appl Hlth Sci, Sao Paulo, SP - Brazil

^[2] Univ Nove Julho UNINOVE, Postgrad Program Rehabil Sci, Sao Paulo, SP - Brazil

^[3] Univ Nove Julho UNINOVE, Sao Paulo, SP - Brazil

^[4] Univ Nove Julho UNINOVE, Dept Pos Grad, Ciencias Reabil, Rua Vergueiro 235-249, BR-01504001 Sao Paulo, SP - Brazil

• Document type: Journal article
• Source: Lasers in Medical Science; v. 33, n. 6, p. 1207-1213, AUG 2018.
• Web of Science Citations: 0

Abstract

The aim of the present study was to evaluate the effects of LLLT prior to muscle injury with and without post-injury irradiation on the expression of isoforms of myosin heavy chain (MyHC), calcineurin (CaN), and myostatin during the repair process. Wistar rats were divided into five groups: control (n = 7); injury (n = 21); LLLT + injury (n = 21); injury + LLLT (n = 21), and LLLT + injury + LLLT (n = 21). Cryoinjury was performed on the tibialis anterior (TA) muscle. The injured groups were euthanized at 3, 7, and 14 days after injury. LLLT was performed using an infrared laser (780 nm) with the following parameters: 10 J/cm(2), 40 mW, 10 s per point, 8 points, and 3.2 J of total energy. At the end of each period, the TA muscle was removed for the analysis of MyHC, CaN, and myostatin gene expression using real-time PCR. The data were tested statistically by Kruskal-Wallis with Dunn's post hoc test (p < 0.05). The results demonstrated that prior irradiation reduced the mRNA expression of all proteins at 3 days. Post irradiation reduced the mRNA expression of MyHC-1, MyHC-2a, MyHC-2b, and CaN at 7 days. Prior irradiation combined with post-injury irradiation reduced the mRNA expression of MyHC-2x and CaN at 14 days and increased the mRNA expression of myostatin in the same period. In conclusion, different protocols of photobiomodulation can modulate the expression of the different isoforms of MyHC, CaN, and myostatin during the repair process. It is noteworthy that the combination of the prior and post-injury irradiation was the protocol that most promoted changes in the final phase of the repair process. (AU)

• FAPESP's process: 17/06461-0 - Effects of laser therapy and therapeutic ultrasound on the protein expression of myosin heavy chain isoforms during the skeletal muscle repair of rats
• Grantee: Weslley Wallace de Alcântara Araujo Amorim
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 14/12381-1 - Comparative analysis of the effect of previous laser therapy associated or not to post treatment on the modulation of inflammatory cytokines, oxidative stress and the determination of muscle fiber type after acute injury
• Grantee: Raquel Agnelli Mesquita Ferrari
• Support Opportunities: Regular Research Grants
• FAPESP's process: 13/21540-3 - Comparative effect of low-level laser therapy and low intensity ultrasound on myofiber phenotypes, oxidative stress and proteins during muscle repair
• Grantee: Agnelo Neves Alves
• Support Opportunities: Scholarships in Brazil - Doctorate