Prevalence and molecular features of ESBL/pAmpC-producing Enterobacteriaceae in healthy and diseased companion animals in Brazil

Autor(es)

Melo, Luana C. ^{[1, 2]} ; Oresco, Cintia ^[1] ; Leigue, Lucianne ^[1] ; Netto, Hildebrando M. ^[3] ; Melville, Priscilla A. ^[4] ; Benites, Nilson R. ^[4] ; Saras, Estelle ^[2] ; Haenni, Marisa ^[2] ; Lincopan, Nilton ^[1] ; Madec, Jean-Yves ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, Sao Paulo - Brazil

^[2] Univ Claude Bernard Lyon 1, Unite Antibioresistance & Virulence Bacteriennes, ANSES Lab Lyon, Lyon - France

^[3] Ctr Zoonosis Control, Sao Paulo - Brazil

^[4] Univ Sao Paulo, Dept Prevent Vet Med & Anim Hlth, Fac Vet Med, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Veterinary Microbiology; v. 221, p. 59-66, JUL 2018.
• Citações Web of Science: 4

Resumo

Extended-spectrum beta-lactamase (ESBL)-and plasmid-mediated AmpC (pAmpC)-carrying Enterobacteriaceae have widely disseminated in human, animal and environmental reservoirs. Pets have been recognized as a source of ESBL/pAmpC worldwide, and are possibly also a source of human contamination. The aim of this study was to document to what extent cats and dogs may act as a driving force in the spread of ESBLs and pAmpCs in Brazil. A total of 113 healthy stray cats and dogs and 74 sick pets were sampled, and extended-spectrum cephalosporinresistant Enterobacteriaceae (ESC-R) were detected in 28/113 (24.8%) and 8/74 (10.8%) tested animals, respectively. Different Enterobacteriaceae isolates (mostly E. coli), a large number of E. coli clones (with ST90, ST457, ST973 and ST2541 being predominant), and several ESBL/pAmpC genes and plasmids were characterized, highlighting the ability of stray and pet cats and dogs to further spread a wide range of ESC-resistance determinants. The ESBL phenotype was due to the bla(CTX-M-2) and bla(CTX-M-8) genes, as found in human epidemiology in Brazil, but bla(CTX.M.9) and bla(CTX-M-15) were also identified. The pAmpC phenotype was systematically due to the presence of the bla(CMY-2) gene, mostly carried by Incl1 ST12 plasmids. Our results showed that pets can be considered a significant reservoir of multidrug-resistant bacteria in Brazil. This is especially true for healthy stray dogs that displayed the highest prevalence (24.8%) of ESBLs/pAmpC resistance determinants, which can then be further spread both to the environment and to other animals or humans by contact. (AU)

• Processo FAPESP: 16/08593-9 - Pan-resistoma de Klebsiella pneumoniae e Escherichia coli produtoras de beta-lactamases (KPC-2, CTX-M-8, CTX-M-15) endêmicas no Brasil
• Beneficiário: Nilton Erbet Lincopan Huenuman
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/04725-0 - Monitoramento de bactérias Gram-negativas multirresistentes de importância médica (humana e veterinária): impacto clínico/ambiental e desenvolvimento de alternativas terapêuticas e produtos de inovação tecnológica
• Beneficiário: Nilton Erbet Lincopan Huenuman
• Modalidade de apoio: Auxílio à Pesquisa - Regular