Effect of low-level laser therapy on the healing of sites grafted with coagulum, deproteinized bovine bone, and biphasic ceramic made of hydroxyapatite and -tricalcium phosphate. In vivo study in rats

Autor(es)

de Oliveira, Guilherme J. P. L. ^[1] ; Aroni, Mauricio A. T. ^[1] ; Medeiros, Marcell C. ^[1] ; Marcantonio, Jr., Elcio ^[1] ; Marcantonio, Rosemary A. C. ^[1]

Afiliação do(s) autor(es)

^[1] State Univ Sao Paulo Univ Estadual Paulista UNESP, Sch Dent Araraquara, Dept Diag & Surg, Humaita St 1680, BR-14801930 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Lasers in Surgery and Medicine; v. 50, n. 6, p. 651-660, AUG 2018.
• Citações Web of Science: 1

Resumo

ObjectiveThe aim of this study was to evaluate the effect of low-level laser therapy (LLLT) on the healing of biomaterial graft areas (i.e., coagulum, deproteinized bovine bone, and biphasic ceramics comprising hydroxyapatite and -tricalcium phosphate). Material and MethodsNinety rats were divided into two groups according to laser irradiation use ( 808nm, 100mW, approximate to 600m, seven sessions with 28J of irradiation dose in total): a laser group and a control group. Each of these groups was divided into three subgroups of 15 animals each according to the type of biomaterial used: Coagulum (COA), deproteinized bovine bone (DBB), and hydroxyapatite/-tricalcium phosphate (HA/TCP). Biomaterials were inserted into Teflon domes, and these domes were grafted to the lateral aspect of the mandibular branch of the rats. The animals were sacrificed after 30, 60, and 90 days. Scarring patterns were evaluated by microtomography and histometry. The expression levels of BMP2, osteocalcin (OCN), and alkaline phosphatase (ALP) were evaluated by immunohistochemistry. The mRNA expression levels of ALP, BMP2, Jagged1, Osterix, Runx2, and TGF1 were determined by RT-qPCR. ResultsThe animals treated with LLLT exhibited increased mineralized tissues and bone, particularly after 90 days. These increases were associated with increased BMP2, OCN, and ALP protein expression and ALP, BMP2, and Jagged1 mRNA expression. ConclusionLLLT improved the osteoconductive potential of DBB and HA/TCP grafts and bone formation in ungrafted areas. Lasers Surg. Med. 50:651-660, 2018. (c) 2018 Wiley Periodicals, Inc. (AU)

• Processo FAPESP: 13/24318-0 - Efeito da irradiação com laser de baixa intensidade (830nm) sobre a osseointegração de implantes em regiões enxertadas com diferentes biomateriais osteocondutores
• Beneficiário: Guilherme José Pimentel Lopes de Oliveira
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado