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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Effect of low-level laser therapy on the healing of sites grafted with coagulum, deproteinized bovine bone, and biphasic ceramic made of hydroxyapatite and -tricalcium phosphate. In vivo study in rats

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Author(s):
de Oliveira, Guilherme J. P. L. [1] ; Aroni, Mauricio A. T. [1] ; Medeiros, Marcell C. [1] ; Marcantonio, Jr., Elcio [1] ; Marcantonio, Rosemary A. C. [1]
Total Authors: 5
Affiliation:
[1] State Univ Sao Paulo Univ Estadual Paulista UNESP, Sch Dent Araraquara, Dept Diag & Surg, Humaita St 1680, BR-14801930 Sao Paulo - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Lasers in Surgery and Medicine; v. 50, n. 6, p. 651-660, AUG 2018.
Web of Science Citations: 1
Abstract

ObjectiveThe aim of this study was to evaluate the effect of low-level laser therapy (LLLT) on the healing of biomaterial graft areas (i.e., coagulum, deproteinized bovine bone, and biphasic ceramics comprising hydroxyapatite and -tricalcium phosphate). Material and MethodsNinety rats were divided into two groups according to laser irradiation use ( 808nm, 100mW, approximate to 600m, seven sessions with 28J of irradiation dose in total): a laser group and a control group. Each of these groups was divided into three subgroups of 15 animals each according to the type of biomaterial used: Coagulum (COA), deproteinized bovine bone (DBB), and hydroxyapatite/-tricalcium phosphate (HA/TCP). Biomaterials were inserted into Teflon domes, and these domes were grafted to the lateral aspect of the mandibular branch of the rats. The animals were sacrificed after 30, 60, and 90 days. Scarring patterns were evaluated by microtomography and histometry. The expression levels of BMP2, osteocalcin (OCN), and alkaline phosphatase (ALP) were evaluated by immunohistochemistry. The mRNA expression levels of ALP, BMP2, Jagged1, Osterix, Runx2, and TGF1 were determined by RT-qPCR. ResultsThe animals treated with LLLT exhibited increased mineralized tissues and bone, particularly after 90 days. These increases were associated with increased BMP2, OCN, and ALP protein expression and ALP, BMP2, and Jagged1 mRNA expression. ConclusionLLLT improved the osteoconductive potential of DBB and HA/TCP grafts and bone formation in ungrafted areas. Lasers Surg. Med. 50:651-660, 2018. (c) 2018 Wiley Periodicals, Inc. (AU)

FAPESP's process: 13/24318-0 - Effect of the low level laser irradiation (830nm) on the implants osseointegration in grafted areas with different osteoconductors biomaterials
Grantee:Guilherme José Pimentel Lopes de Oliveira
Support Opportunities: Scholarships in Brazil - Post-Doctoral