Cytotoxicity Assessment of a Poly(methyl methacrylate) Synthesized for the Direct Fabrication of Bone Tissues

Autor(es)

Linan, Lamia Zuniga ^[1] ; Nascimento Lima, Nadson Murilo ^[1] ; Benatti, Carol ^[1] ; Xavier, Mariana ^[1] ; Rodrigues, Ana A. ^[1] ; Manenti, Flavio ^[2] ; Jardini, Andre ^[1] ; Maciel Filho, Rubens ^[1] ; Gilioli, Rovilson ^[3]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Dept Desenvolvimento Proc & Prod, Campinas, SP - Brazil

^[2] Politecn Milan, Dipartamento Chim Mat & Engn Chim Giulio Natta, Milan, Lombardia - Italy

^[3] Univ Estadual Campinas, Ctr Multidisciplinar Invest Biol Area Ciencia Ani, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Archives of Biology and Technology; v. 61, 2018.
• Citações Web of Science: 1

Resumo

ABSTRACT A cytotoxicity study is performed on a poly(methyl methacrylate) polymer (PMMA) to be used for the fabrication of bone tissue by Rapid Prototyping (RP). The solution polymerization is conducted in a pilot plant reactor using more appropriated reagents in consideration of the medical application. Moreover, the polymer is efficiently handled to avoid the side effect of the monomer, reducing the concentration of this specie to 287,731 µg MMA/kg PMMA. The cytotoxicity of the polymer is determined through growth monitoring, adherence and morphology of L-929 cells. Additionally, MTT and LIVE/DEAD tests are performed. The results showed continuous and progressive growth of the cells on the surface of the specimens. Moreover, the material did not influence on the viability of mesenchymal cells and inverted fluorescence microscopy images showed a polyanionic dye calcein well retained in the cells in contact with the PMMA as well as the negative control after 72 hours. Thus, the polymer was efficiently synthesized and handled for the expected demands. (AU)

• Processo FAPESP: 11/09631-8 - Estudo e modelagem do processo de produção do polimetilmetacrilato para a fabricação de tecido ósseo artificial
• Beneficiário: Rubens Maciel Filho
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 08/57860-3 - BIOFABRIS - Instituto de Biofabricação
• Beneficiário: Rubens Maciel Filho
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 10/16952-2 - Modelagem e controle ótimo por lógica fuzzy de processos de produção de biomateriais
• Beneficiário: Nádson Murilo Nascimento Lima
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 09/09092-0 - Estudo e modelagem do processo de produção do polimetilmetacrilato para a fabricação de tecido ósseo artificial
• Beneficiário: Lamia Zuniga Linan
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado