Effects of myogenic precursor cells (C2C12) transplantation and low-level laser therapy on muscle repair

Autor(es):Mostrar menos -

Andreo, Lucas ^[1] ; Mesquita-Ferrari, Raquel A. ^{[2, 1]} ; Ribeiro, Beatriz G. ^[2] ; Benitte, Adriana ^[3] ; Nogueira, Tatiane de Fatima ^[3] ; Franca, Cristiane M. ^[1] ; Teixeira da Silva, Daniela de Fatima ^[1] ; Bussadori, Sandra K. ^{[2, 1]} ; Fernandes, Kristianne P. S. ^[1] ; Correa, Fernanda I. ^[2] ; Correa, Joao C. F. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Nove Julho UNINOVE, Postgrad Program Biophoton Appl Hlth Sci, Rua Vergueiro 235-249, BR-01504001 Sao Paulo, SP - Brazil

^[2] UNINOVE, Postgrad Program Rehabil Sci, Sao Paulo, SP - Brazil

^[3] UNINOVE, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Lasers in Surgery and Medicine; v. 50, n. 7, p. 781-791, SEP 2018.
• Citações Web of Science: 1

Resumo

ObjectiveThe aim of the present study was to evaluate the effects of myoblast inoculation in combination with photobiomodulation therapy (PBMT) on skeletal muscle tissue following injury. Materials and MethodsSixty-five Wistar rats were divided into five groups: Controlanimals not submitted to any procedure; Injurycryoinjury of the tibialis anterior muscle; HBSSanimals submitted to cryoinjury and intramuscular Hank's Balanced Salt Solution; Injury+Cellsanimals submitted to cryoinjury, followed by myogenic precursor cells (C2C12) transplantation; Injury+Cells+LLLTanimals submitted to cryoinjury, followed by myogenic precursor cells (C2C12) transplantation and PBMT (780nm, 40mW, 3.2J in 8 points). The periods analyzed were 1, 3, and 7 days. The tibialis anterior muscle was harvest for histological analysis, collagen analysis, and immunolabeling of macrophages. ResultsNo differences were found between the HBSS group and injury group. The Injury+Cells group exhibited an increase of inflammatory cells and immature fibers as well as a decrease in the number of macrophages on Day 1. The Injury+Cells+LLLT group exhibited a decrease in myonecrosis and inflammatory infiltrate at 7 days, but an increase in inflammatory infiltrate at 1 and 3 days as well as an increase in blood vessels at 3 and 7 days, an increase in macrophages at 3 days and better collagen organization at 7 days. ConclusionCell transplantation combined with PBMT led to an increase in the number of blood vessels, a reduction in myonecrosis and total inflammatory cells as well as better organization of collagen fibers during the skeletal muscle repair process. Lasers Surg. Med. 50:781-791, 2018. (c) 2018 Wiley Periodicals, Inc. (AU)

• Processo FAPESP: 14/12381-1 - Análise comparativa do efeito da laserterapia prévia associada ou não ao tratamento pós sobre a modulação de citocinas inflamatórias, do estresse oxidativo e a determinação do tipo de fibra muscular após lesão aguda
• Beneficiário: Raquel Agnelli Mesquita Ferrari
• Modalidade de apoio: Auxílio à Pesquisa - Regular