Effects of myogenic precursor cells (C2C12) transplantation and low-level laser therapy on muscle repair

Author(s):Show less -

Andreo, Lucas ^[1] ; Mesquita-Ferrari, Raquel A. ^{[2, 1]} ; Ribeiro, Beatriz G. ^[2] ; Benitte, Adriana ^[3] ; Nogueira, Tatiane de Fatima ^[3] ; Franca, Cristiane M. ^[1] ; Teixeira da Silva, Daniela de Fatima ^[1] ; Bussadori, Sandra K. ^{[2, 1]} ; Fernandes, Kristianne P. S. ^[1] ; Correa, Fernanda I. ^[2] ; Correa, Joao C. F. ^[2]

Affiliation

^[1] Univ Nove Julho UNINOVE, Postgrad Program Biophoton Appl Hlth Sci, Rua Vergueiro 235-249, BR-01504001 Sao Paulo, SP - Brazil

^[2] UNINOVE, Postgrad Program Rehabil Sci, Sao Paulo, SP - Brazil

^[3] UNINOVE, Sao Paulo, SP - Brazil

• Document type: Journal article
• Source: Lasers in Surgery and Medicine; v. 50, n. 7, p. 781-791, SEP 2018.
• Web of Science Citations: 1

Abstract

ObjectiveThe aim of the present study was to evaluate the effects of myoblast inoculation in combination with photobiomodulation therapy (PBMT) on skeletal muscle tissue following injury. Materials and MethodsSixty-five Wistar rats were divided into five groups: Controlanimals not submitted to any procedure; Injurycryoinjury of the tibialis anterior muscle; HBSSanimals submitted to cryoinjury and intramuscular Hank's Balanced Salt Solution; Injury+Cellsanimals submitted to cryoinjury, followed by myogenic precursor cells (C2C12) transplantation; Injury+Cells+LLLTanimals submitted to cryoinjury, followed by myogenic precursor cells (C2C12) transplantation and PBMT (780nm, 40mW, 3.2J in 8 points). The periods analyzed were 1, 3, and 7 days. The tibialis anterior muscle was harvest for histological analysis, collagen analysis, and immunolabeling of macrophages. ResultsNo differences were found between the HBSS group and injury group. The Injury+Cells group exhibited an increase of inflammatory cells and immature fibers as well as a decrease in the number of macrophages on Day 1. The Injury+Cells+LLLT group exhibited a decrease in myonecrosis and inflammatory infiltrate at 7 days, but an increase in inflammatory infiltrate at 1 and 3 days as well as an increase in blood vessels at 3 and 7 days, an increase in macrophages at 3 days and better collagen organization at 7 days. ConclusionCell transplantation combined with PBMT led to an increase in the number of blood vessels, a reduction in myonecrosis and total inflammatory cells as well as better organization of collagen fibers during the skeletal muscle repair process. Lasers Surg. Med. 50:781-791, 2018. (c) 2018 Wiley Periodicals, Inc. (AU)

• FAPESP's process: 14/12381-1 - Comparative analysis of the effect of previous laser therapy associated or not to post treatment on the modulation of inflammatory cytokines, oxidative stress and the determination of muscle fiber type after acute injury
• Grantee: Raquel Agnelli Mesquita Ferrari
• Support Opportunities: Regular Research Grants