Using FT-IR spectroscopy for the identification of the T-cruzi, T-rangeli, and the L-chagasi species

Autor(es)

Aguiar, J. C. ^[1] ; Mittmann, J. ^[2] ; Caetano Junior, P. C. ^[3] ; Raniero, L. ^[3]

Afiliação do(s) autor(es)

^[1] Inst Fed Educ Ciencia & Tecnol Tocantins, Povoado Santa Teresa Km 05 Zona Rural, BR-77950000 Araguatins, Tocantins - Brazil

^[2] Univ Fed Santa Catarina, CCB, Dept Microbiol Imunol & Parasitol MIP, Lab Protozool, Bloco A Cx Postal 476, BR-88040970 Florianopolis, SC - Brazil

^[3] Univ Vale Paraiba, Lab Nanossensores, IP&D, Shishima Hifumi Ave 2911, BR-12244000 Sao Jose Dos Campos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Experimental Parasitology; v. 192, p. 46-51, SEP 2018.
• Citações Web of Science: 0

Resumo

The cross-reaction in the diagnosis results is a serious problem, leading to an incorrect treatment and several injuries to patients. The Trypanosoma rangeli and Trypanosoma cruzi belong to the genus Trypanosoma, but the Trypanosoma rangeli is a non-pathogenic parasite to humans. While Trypanosoma cruzi is the etiological agent of Chagas' disease, which affects circa 2-3 million people and more than 6000 deaths annually in Brazil. The Leishmania chagasi causes infectious disease known as visceral leishmaniasis. This diseases have in common the crossed antigenic reaction promoted by serological tests and its differentiation is relevant for epidemiological studies and clinical practice. In this study the Fourier Transform Infrared (FT-IR) Spectroscopy was used to differentiate these microorganisms, which were cultivated and the spectra analyzed. Data analysis were performed by Gaussian curve fitting and multivariate statistical analysis. The cluster analysis have shown four specific regions to identify the microorganisms. The first three PCs of principal component analysis associated to linear discriminant were able to classify 95.6% of the parasites using cross-validation. The curve fitting method showed the quantitative differentiation among L. chagasi, T. cruzi, and T. rangeli species in the vibrational regions of polysaccharides, amide III, lipid esters, and fatty acid. (AU)

• Processo FAPESP: 09/09559-5 - Nanossonda de ouro no diagnóstico do Paracoccidioides brasiliensis
• Beneficiário: Leandro José Raniero
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/17404-7 - Terapia fotodinâmica associada a nanossondas para o tratamento do câncer
• Beneficiário: Leandro José Raniero
• Modalidade de apoio: Auxílio à Pesquisa - Regular