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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Using FT-IR spectroscopy for the identification of the T-cruzi, T-rangeli, and the L-chagasi species

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Author(s):
Aguiar, J. C. [1] ; Mittmann, J. [2] ; Caetano Junior, P. C. [3] ; Raniero, L. [3]
Total Authors: 4
Affiliation:
[1] Inst Fed Educ Ciencia & Tecnol Tocantins, Povoado Santa Teresa Km 05 Zona Rural, BR-77950000 Araguatins, Tocantins - Brazil
[2] Univ Fed Santa Catarina, CCB, Dept Microbiol Imunol & Parasitol MIP, Lab Protozool, Bloco A Cx Postal 476, BR-88040970 Florianopolis, SC - Brazil
[3] Univ Vale Paraiba, Lab Nanossensores, IP&D, Shishima Hifumi Ave 2911, BR-12244000 Sao Jose Dos Campos, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Experimental Parasitology; v. 192, p. 46-51, SEP 2018.
Web of Science Citations: 0
Abstract

The cross-reaction in the diagnosis results is a serious problem, leading to an incorrect treatment and several injuries to patients. The Trypanosoma rangeli and Trypanosoma cruzi belong to the genus Trypanosoma, but the Trypanosoma rangeli is a non-pathogenic parasite to humans. While Trypanosoma cruzi is the etiological agent of Chagas' disease, which affects circa 2-3 million people and more than 6000 deaths annually in Brazil. The Leishmania chagasi causes infectious disease known as visceral leishmaniasis. This diseases have in common the crossed antigenic reaction promoted by serological tests and its differentiation is relevant for epidemiological studies and clinical practice. In this study the Fourier Transform Infrared (FT-IR) Spectroscopy was used to differentiate these microorganisms, which were cultivated and the spectra analyzed. Data analysis were performed by Gaussian curve fitting and multivariate statistical analysis. The cluster analysis have shown four specific regions to identify the microorganisms. The first three PCs of principal component analysis associated to linear discriminant were able to classify 95.6% of the parasites using cross-validation. The curve fitting method showed the quantitative differentiation among L. chagasi, T. cruzi, and T. rangeli species in the vibrational regions of polysaccharides, amide III, lipid esters, and fatty acid. (AU)

FAPESP's process: 09/09559-5 - Gold nano-probe for Paracoccidioides brasiliensis diagnoses
Grantee:Leandro José Raniero
Support Opportunities: Regular Research Grants
FAPESP's process: 13/17404-7 - Photodynamic therapy associated with nanoprobes for treating cancer
Grantee:Leandro José Raniero
Support Opportunities: Regular Research Grants