Zika-virus-infected human full-term placental explants display pro-inflammatory responses and undergo apoptosis

Autor(es):Mostrar menos -

Ribeiro, Milene Rocha ^{[1, 2]} ; Moreli, Jusciele Brogin ^[3] ; Marques, Rafael Elias ^[4] ; Papa, Michelle Premazzi ^[5] ; Meuren, Lana Monteiro ^[5] ; Rahal, Paula ^[1] ; de Arruda, Luciana Barros ^[5] ; Oliani, Antonio Helio ^[2] ; Mos Vaz Oliani, Denise Cristina ^[2] ; Oliani, Sonia Maria ^[1] ; Narayanan, Aarthi ^[6] ; Nogueira, Mauricio Lacerda ^[2]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Sch Biosci Humanities & Exact Sci, Dept Biol, Sao Jose Do Rio Preto, SP - Brazil

^[2] Sao Jose do Rio Preto Sch Med FAMERP, Dept Dermatol Infect & Parasit Dis, Virol Res Lab, Sao Jose Do Rio Preto, SP - Brazil

^[3] Fed Univ Sao Paulo Unifesp, Sao Paulo, SP - Brazil

^[4] Natl Ctr Res Energy & Mat CNPEM, Brazilian Biosci Natl Lab LNBio, Campinas, SP - Brazil

^[5] Fed Univ Rio de Janeiro UFRJ, Paulo de Goes Dept Microbiol, Rio De Janeiro - Brazil

^[6] George Mason Univ, Fairfax, VA 22030 - USA

• Tipo de documento: Artigo Científico
• Fonte: ARCHIVES OF VIROLOGY; v. 163, n. 10, p. 2687-2699, OCT 2018.
• Citações Web of Science: 3

Resumo

Zika virus (ZIKV) is a flavivirus that has been highly correlated with the development of neurological disorders and other malformations in newborns and stillborn fetuses after congenital infection. This association is supported by the presence of ZIKV in the fetal brain and amniotic fluid, and findings suggest that infection of the placental barrier is a critical step for fetal ZIKV infection in utero. Therefore, relevant models to investigate the interaction between ZIKV and placental tissues are essential for understanding the pathogenesis of Zika syndrome. In this report, we demonstrate that explant tissue from full-term human placentas sustains a productive ZIKV infection, though the results depend on the strain. Viral infection was found to be associated with pro-inflammatory cytokine expression and apoptosis of the infected tissue, and these findings confirm that placental explants are targets of ZIKV replication. We propose that human placental explants are useful as a model for studying ZIKV infection ex vivo. (AU)

• Processo FAPESP: 13/21719-3 - Estudo epidemiológico da dengue (sorotipos 1 a 4) em coorte prospectiva de São José do Rio Preto, São Paulo, Brasil, durante 2014 a 2018
• Beneficiário: Maurício Lacerda Nogueira
• Modalidade de apoio: Auxílio à Pesquisa - Temático