Nicotine and sleep deprivation: impact on pain sensitivity and immune modulation in rats

Autor(es)

Hirotsu, Camila ^[1] ; Pedroni, Matheus Negrao ^[1] ; Berro, Lais Fernanda ^[2] ; Tufik, Sergio ^[1] ; Andersen, Monica Levy ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Dept Psychobiol, Sao Paulo - Brazil

^[2] Univ Mississippi, Med Ctr, Dept Psychiat & Human Behav, Jackson, MS 39216 - USA

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 8, SEP 14 2018.
• Citações Web of Science: 1

Resumo

Repeated nicotine administration has been associated with increased paradoxical sleep in rats and antinociceptive properties, whereas paradoxical sleep deprivation (PSD) elicits pronociceptive and inflammatory responses. Thus, we aimed to evaluate the effect of repeated nicotine administration and its withdrawal combined with PSD on pain sensitivity and inflammatory markers. Sixty adult male Wistar rats were subjected to repeated injections of saline (SAL) or nicotine (NIC) for 12 days or 7 days of nicotine followed by acute mecamylamine administration on day 8 to precipitate nicotine abstinence (ABST). On day 9, the animals were submitted to PSD for 72 h or remained in control condition (CTRL); on day 12, thermal pain threshold was assessed by the hot plate test. PSD significantly decreased the latency to paw withdrawal in all groups compared to their respective controls. ABST-PSD animals presented higher levels of interleukin (IL)-6 compared to all groups, except ABST-CTRL. After adjustment for weight loss, IL-6, IL-4 and tumor necrosis factor alpha, ABST-PSD was associated with the lowest pain threshold. Nicotine and IL-4 levels were predictors of higher pain threshold. Hyperalgesia induced by PSD prevailed over the antinociceptive action of nicotine, while the association between PSD and ABST synergistically increased IL-6 concentrations and decreased pain threshold. (AU)

• Processo FAPESP: 14/15259-2 - Epidemiologia do sono na cidade de São Paulo: seguimento dos voluntários do estudo de base EPISONO
• Beneficiário: Camila Hirotsu
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado