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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Surface-enhanced Raman spectroscopy and MCR-ALS for the selective sensing of urinary adenosine on filter paper

Texto completo
Autor(es):
Villa, Javier E. L. [1] ; Pasquini, Celio [1] ; Poppi, Ronei J. [1]
Número total de Autores: 3
Afiliação do(s) autor(es):
[1] Univ Estadual Campinas, Inst Chem, POB 6154, BR-13081970 Campinas, SP - Brazil
Número total de Afiliações: 1
Tipo de documento: Artigo Científico
Fonte: Talanta; v. 187, p. 99-105, SEP 1 2018.
Citações Web of Science: 1
Resumo

Adenosine is a purine nucleoside that is present in all human cells and is essential for regulating certain physiological activities in tissues and organs. Since adenosine is considered to be a potential cancer biomarker in urine, its determination may be crucial for the early diagnosis and non-invasive monitoring of cancer. Herein, we present a label-free method to quantify urinary adenosine using surface-enhanced Raman spectroscopy (SERS) and multivariate curve resolution-alternating least squares (MCR-ALS). Ring-oven preconcentration and direct deposition of monodisperse gold nanoparticles on filter paper were employed to improve the sampling efficiency. Further, MCR-ALS (assessed with and without a correlation constraint), the standard addition method and pH controls were combined to compensate for the matrix effect and to address overlapping bands in the analysis of human urine samples. As a result, the proposed method showed to be sensitive (LOD varying between 3.8 and 4.9 mu mol L-1, S/R = 3), reproducible (RSD less than +/- 15%), and selective over other nucleosides (guanosine, cytidine, thymidine and uridine) and unknown interferences (second-order advantage). This is the first report of a SERS-chemometric method applied to urinary adenosine sensing at physiologically relevant concentrations, with minimal sample preparation, and has strong potential to be a valuable tool in cancer research. (AU)

Processo FAPESP: 14/50951-4 - INCT 2014: Tecnologias Analíticas Avançadas
Beneficiário:Celio Pasquini
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 08/57808-1 - Instituto Nacional de Ciências e Tecnologias Analíticas Avançadas - INCTAA
Beneficiário:Celio Pasquini
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 08/57805-2 - Instituto de Bioanalítica
Beneficiário:Lauro Tatsuo Kubota
Linha de fomento: Auxílio à Pesquisa - Temático