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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Surface-enhanced Raman spectroscopy and MCR-ALS for the selective sensing of urinary adenosine on filter paper

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Villa, Javier E. L. [1] ; Pasquini, Celio [1] ; Poppi, Ronei J. [1]
Total Authors: 3
[1] Univ Estadual Campinas, Inst Chem, POB 6154, BR-13081970 Campinas, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Talanta; v. 187, p. 99-105, SEP 1 2018.
Web of Science Citations: 1

Adenosine is a purine nucleoside that is present in all human cells and is essential for regulating certain physiological activities in tissues and organs. Since adenosine is considered to be a potential cancer biomarker in urine, its determination may be crucial for the early diagnosis and non-invasive monitoring of cancer. Herein, we present a label-free method to quantify urinary adenosine using surface-enhanced Raman spectroscopy (SERS) and multivariate curve resolution-alternating least squares (MCR-ALS). Ring-oven preconcentration and direct deposition of monodisperse gold nanoparticles on filter paper were employed to improve the sampling efficiency. Further, MCR-ALS (assessed with and without a correlation constraint), the standard addition method and pH controls were combined to compensate for the matrix effect and to address overlapping bands in the analysis of human urine samples. As a result, the proposed method showed to be sensitive (LOD varying between 3.8 and 4.9 mu mol L-1, S/R = 3), reproducible (RSD less than +/- 15%), and selective over other nucleosides (guanosine, cytidine, thymidine and uridine) and unknown interferences (second-order advantage). This is the first report of a SERS-chemometric method applied to urinary adenosine sensing at physiologically relevant concentrations, with minimal sample preparation, and has strong potential to be a valuable tool in cancer research. (AU)

FAPESP's process: 14/50951-4 - INCT 2014: Advanced Analytical Technologies
Grantee:Celio Pasquini
Support type: Research Projects - Thematic Grants
FAPESP's process: 08/57808-1 - National Institute of Advanced Analytical Science and Technology
Grantee:Celio Pasquini
Support type: Research Projects - Thematic Grants
FAPESP's process: 08/57805-2 - Institute of Bioanalytics
Grantee:Lauro Tatsuo Kubota
Support type: Research Projects - Thematic Grants