QTL detection for growth and latex production in a full-sib rubber tree population cultivated under suboptimal climate conditions

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Feijo Rosa, Joao Ricardo Bachega ^{[1, 2]} ; Mantello, Camila Campos ^{[3, 4]} ; Garcia, Dominique ^{[5, 6]} ; de Souza, Livia Moura ^[3] ; da Silva, Carla Cristina ^[3] ; Gazaffi, Rodrigo ^[7] ; da Silva, Cicero Casimiro ^[8] ; Toledo-Silva, Guilherme ^{[3, 9]} ; Cubry, Philippe ^[10] ; Franco Garcia, Antonio Augusto ^[1] ; de Souza, Anete Pereira ^{[3, 11]} ; Le Guen, Vincent ^{[5, 6]}

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^[1] Univ Sao Paulo, Luiz de Queiros Coll Agr ESALQ, Dept Genet, Ave Padua Dias 11, BR-13400970 Pircacicaba, SP - Brazil

^[2] FTS Sementes SA, Ave Newton Slaviero, BR-84043560 Ponta Grossa, PR - Brazil

^[3] Univ Campinas UNICAMP, Mol Biol & Genet Engn Ctr CBMEG, Campinas, SP - Brazil

^[4] NIAB, Huntingdon Rd, Cambridge CB3 0LE - England

^[5] CIRAD, UMR AGAP, F-34398 Montpellier - France

^[6] Univ Montpellier, AGAP, CIRAD, INRA, INRIA, Montpellier SupAgro, Montpellier - France

^[7] Fed Univ Sao Carlos UFSCAR, Dept Biotechnol & Vegetal & Anim Prod, Ctr Agron Sci, BR-13604900 Araras, SP - Brazil

^[8] Plantat E Michelin, R&D Dept, Rua Joao de Barro Quadra 22, Lote 16, BR-78790000 Itiquira, MT - Brazil

^[9] Univ Fed Santa Catarina, Dept Biochem, Lab Biomarkers Aquat Contaminat & Immunochem LABC, Florianopolis, SC - Brazil

^[10] IRD, UMR DiADE, 911 Ave Agropolis, BP 64501, F-34394 Montpellier 5 - France

^[11] Univ Campinas UNICAMP, Inst Biol, Dept Plant Biol, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BMC PLANT BIOLOGY; v. 18, OCT 10 2018.
• Citações Web of Science: 3

Resumo

Background: Rubber tree is cultivated in mainly Southeast Asia and is by far the most significant source of natural rubber production worldwide. However, the genetic architecture underlying the primary agronomic traits of this crop has not been widely characterized. This study aimed to identify quantitative trait loci (QTLs) associated with growth and latex production using a biparental population established in suboptimal growth conditions in Brazil. Results: A full-sib population composed of 251 individuals was developed from crossing two high-producing Asiatic rubber tree cultivars, PR 255 and PB 217. This mapping population was genotyped with microsatellite markers from enriched genomic libraries or transcriptome datasets and single-nucleotide polymorphism (SNP) markers, leading to construction of a saturated multipoint integrated genetic map containing 354 microsatellite and 151 SNP markers. Height and circumference measurements repeated over a six-year period and registration of cumulative latex production during six consecutive months on the same individuals allowed in-depth characterization of the genetic values of several growth traits and precocious latex production. Growth traits, circumference and height, were overall positively correlated, whereas latex production was not correlated or even negatively correlated with growth traits. A total of 86 distinct QTLs were identified, most of which were detected for only one trait. Among these QTLs, 15 were linked to more than one phenotypic trait (up to 4 traits simultaneously). Latex production and circumference increments during the last wintering period were associated with the highest numbers of identified QTLs (eleven and nine, respectively), jointly explaining the most significantly observed phenotypic variances (44.1% and 44.4%, respectively). The most important QTL for latex production, located on linkage group 16, had an additive effect of the male parent PB 217 and corresponded to a QTL at the same position detected in a previous study carried out in Thailand for the biparental population RRIM 600 x PB 217. Conclusions: Our results identified a set of significant QTLs for rubber tree, showing that the performance of modern Asiatic cultivars can still be improved and paving the way for further marker-assisted selection, which could accelerate breeding programs. (AU)

• Processo FAPESP: 11/50188-0 - Mapeamento genético molecular em Hevea brasiliensis
• Beneficiário: Camila Campos Mantello
• Linha de fomento: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 09/14068-0 - Desenvolvimento de modelos genético-estatísticos e software para mapeamento de QTLs em cana-de-açúcar
• Beneficiário: Rodrigo Gazaffi
• Linha de fomento: Bolsas no Brasil - Programa Capacitação - Treinamento Técnico
• Processo FAPESP: 12/50491-8 - Avaliação da variabilidade alélica, estrutura populacional e desequilíbrio de ligação no germoplasma ex situ de seringueira (Hevea brasiliensis)
• Beneficiário: Anete Pereira de Souza
• Linha de fomento: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/20447-0 - Análise de transcriptoma de Hevea pauciflora e Hevea benthamiana e identificação de grupos de genes ortólogos para o gênero Hevea
• Beneficiário: Guilherme de Toledo e Silva
• Linha de fomento: Bolsas no Brasil - Pós-Doutorado