Daily Onset of Light and Darkness Differentially Controls Hematopoietic Stem Cell Differentiation and Maintenance

Autor(es):Mostrar menos -

Golan, Karin ^[1] ; Kumari, Anju ^[1] ; Kollet, Orit ^[1] ; Khatib-Massalha, Eman ^[1] ; Subramaniam, Mohana Devi ^[1] ; Ferreira, Zulma S. ^[2] ; Avemaria, Francesca ^[1] ; Rzeszotek, Sylwia ^[3] ; Garcia-Garcia, Andres ^[4] ; Xie, Stephanie ^[5] ; Flores-Figueroa, Eugenia ^{[5, 6]} ; Gur-Cohen, Shiri ^[1] ; Itkin, Tomer ^[1] ; Ludin-Tal, Aya ^[1] ; Massalha, Hassan ^[7] ; Bernshtein, Biana ^[1] ; Ciechanowicz, Andrzej K. ^[8] ; Brandis, Alexander ^[9] ; Mehlman, Tevie ^[9] ; Bhattacharya, Suditi ^[1] ; Bertagna, Mayla ^[1] ; Cheng, Hui ^{[10, 11, 12]} ; Petrovich-Kopitman, Ekaterina ^[9] ; Janus, Tomasz ^[13] ; Kaushansky, Nathali ^[1] ; Cheng, Tao ^{[10, 11, 12]} ; Sagi, Irit ^[14] ; Ratajczak, Mariusz Z. ^[15] ; Mendez-Ferrer, Simon ^[4] ; Dick, John E. ^[5] ; Markus, Regina P. ^[2] ; Lapidot, Tsvee ^[1]

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^[1] Weizmann Inst Sci, Immunol Dept, Rehovot - Israel

^[2] Univ Sao Paulo, Physiol Dept, Sao Paulo - Brazil

^[3] Pomeranian Med Univ, Physiol Dept, Szczecin - Poland

^[4] Univ Cambridge, Haematol Dept, Cambridge - England

^[5] Univ Hlth Network, Princess Margaret Canc Ctr, Toronto, ON - Canada

^[6] Oncol Hosp, Natl Med Ctr Century XXI, IMSS, Oncol Res Unit, Mexico City, DF - Mexico

^[7] Weizmann Inst Sci, Mol Cell Biol Dept, Rehovot - Israel

^[8] Univ Warsaw, Regenerat Med Dept, Warsaw - Poland

^[9] Weizmann Inst Sci, Life Sci Core Facil, Rehovot - Israel

^[10] Chinese Acad Med Sci, Inst Hematol, State Key Lab Expt Hematol, Tianjin - Peoples R China

^[11] Chinese Acad Med Sci, Blood Dis Hosp, Tianjin - Peoples R China

^[12] Peking Union Med Coll, Tianjin - Peoples R China

^[13] Pomeranian Med Univ, Forens Med Dept, Szczecin - Poland

^[14] Weizmann Inst Sci, Biol Regulat Dept, Rehovot - Israel

^[15] Univ Louisville, Stem Cell Inst, Louisville, KY 40292 - USA

• Tipo de documento: Artigo Científico
• Fonte: CELL STEM CELL; v. 23, n. 4, p. 572+, OCT 4 2018.
• Citações Web of Science: 14

Resumo

Hematopoietic stem and progenitor cells (HSPCs) tightly couple maintenance of the bone marrow (BM) reservoir, including undifferentiated long-term repopulating hematopoietic stem cells (LT-HSCs), with intensive daily production of mature leukocytes and blood replenishment. We found two daily peaks of BM HSPC activity that are initiated by onset of light and darkness providing this coupling. Both peaks follow transient elevation of BM norepinephrine and TNF secretion, which temporarily increase HSPC reactive oxygen species (ROS) levels. Lightinduced norepinephrine and TNF secretion augments HSPC differentiation and increases vascular permeability to replenish the blood. In contrast, darkness-induced TNF increases melatonin secretion to drive renewal of HSPCs and LT-HSC potential through modulating surface CD150 and c-Kit expression, increasing COX-2/alpha SMA(+) macrophages, diminishing vascular permeability, and reducing HSPC ROS levels. These findings reveal that light- and darkness-induced daily bursts of norepinephrine, TNF, and melatonin within the BM are essential for synchronized mature blood cell production and HSPC pool repopulation. (AU)

• Processo FAPESP: 15/50166-8 - Efeito do ciclo claro/escuro sobre a produção de células sanguíneas e imunológicas por células tronco da medula espinhal em condições de homeostase e inflamação
• Beneficiário: Regina Pekelmann Markus
• Modalidade de apoio: Auxílio à Pesquisa - Regular