Opportunities for green microextractions in comprehensive two-dimensional gas chromatography / mass spectrometry-based metabolomics - A review

Autor(es)

Belinato de Souza, Joao R. ^{[1, 2]} ; Dias, Fernanda F. G. ^{[1, 2]} ; Caliman, Jaqueline D. ^{[1, 2]} ; Augusto, Fabio ^{[1, 2]} ; Hantao, Leandro W. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Inst Chem, BR-13083970 Campinas, SP - Brazil

^[2] Natl Inst Sci & Technol Bioanal INCTBio, BR-13083970 Campinas, SP - Brazil

• Tipo de documento: Artigo de Revisão
• Fonte: Analytica Chimica Acta; v. 1040, p. 1-18, DEC 21 2018.
• Citações Web of Science: 6

Resumo

Microextractions have become an attractive class of techniques for metabolomics. The most popular technique is solid-phase microextraction that revolutionized the field of modern sample preparation in the early nineties. Ever since this milestone, microextractions have taken on many principles and formats comprising droplets, fibers, membranes, needles, and blades. Sampling devices may be customized to impart exhaustive or equilibrium-based characteristics to the extraction method. Equilibrium-based approaches may rely on additional methods for calibration, such as diffusion-based or on-fiber kinetic calibration to improve bioanalysis. In addition, microextraction-based methods may enable minimally invasive sampling protocols and measure the average free concentration of analytes in heterogeneous multiphasic biological systems. On-fiber derivatization has evidenced new opportunities for targeted and untargeted analysis in metabolomics. All these advantages have highlighted the potential of microextraction techniques for in vivo and on-site sampling and sample preparation, while many opportunities are still available for laboratory protocols. In this review, we outline and discuss some of the most recent applications using microextractions techniques for comprehensive two-dimensional gas chromatography-based metabolomics, including potential research opportunities. (C) 2018 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 11/50390-4 - Estudo biomarcadores de resistência a patógenos em espécies vegetais de interesse comercial através de cromatografia gasosa bidimensional abrangente
• Beneficiário: Leandro Wang Hantao
• Linha de fomento: Bolsas no Brasil - Doutorado
• Processo FAPESP: 16/20547-2 - Caracterização do metaboloma de microorganismos anti-fitopatógenos por cromatografia gasosa multidimensional e espectrometria de massas simples e em Tandem(GC×GC-QMS e GC×GC-MS/MS)
• Beneficiário: Joao Raul Belinato de Souza
• Linha de fomento: Bolsas no Brasil - Doutorado
• Processo FAPESP: 11/51896-9 - Aplicações de cromatografia gasosa bidimensional abrangente na pesquisa de biomarcadores de resistência a patógenos em vegetais
• Beneficiário: Fabio Augusto
• Linha de fomento: Auxílio à Pesquisa - Regular
• Processo FAPESP: 15/05059-9 - Desenvolvimento e avaliação de moduladores microfluídicos planares e livre de consumíveis para cromatografia gasosa bidimensional abrangente
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• Linha de fomento: Auxílio à Pesquisa - Regular
• Processo FAPESP: 17/25490-1 - Síntese e aplicação de líquidos iônicos no estudo de processos fermentativos de interesse comercial
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• Linha de fomento: Auxílio à Pesquisa - Regular