Opportunities for green microextractions in comprehensive two-dimensional gas chromatography / mass spectrometry-based metabolomics - A review

Author(s)

Belinato de Souza, Joao R. ^{[1, 2]} ; Dias, Fernanda F. G. ^{[1, 2]} ; Caliman, Jaqueline D. ^{[1, 2]} ; Augusto, Fabio ^{[1, 2]} ; Hantao, Leandro W. ^[1]

Affiliation

^[1] Univ Estadual Campinas, Inst Chem, BR-13083970 Campinas, SP - Brazil

^[2] Natl Inst Sci & Technol Bioanal INCTBio, BR-13083970 Campinas, SP - Brazil

• Document type: Review article
• Source: Analytica Chimica Acta; v. 1040, p. 1-18, DEC 21 2018.
• Web of Science Citations: 6

Abstract

Microextractions have become an attractive class of techniques for metabolomics. The most popular technique is solid-phase microextraction that revolutionized the field of modern sample preparation in the early nineties. Ever since this milestone, microextractions have taken on many principles and formats comprising droplets, fibers, membranes, needles, and blades. Sampling devices may be customized to impart exhaustive or equilibrium-based characteristics to the extraction method. Equilibrium-based approaches may rely on additional methods for calibration, such as diffusion-based or on-fiber kinetic calibration to improve bioanalysis. In addition, microextraction-based methods may enable minimally invasive sampling protocols and measure the average free concentration of analytes in heterogeneous multiphasic biological systems. On-fiber derivatization has evidenced new opportunities for targeted and untargeted analysis in metabolomics. All these advantages have highlighted the potential of microextraction techniques for in vivo and on-site sampling and sample preparation, while many opportunities are still available for laboratory protocols. In this review, we outline and discuss some of the most recent applications using microextractions techniques for comprehensive two-dimensional gas chromatography-based metabolomics, including potential research opportunities. (C) 2018 Elsevier B.V. All rights reserved. (AU)

• FAPESP's process: 16/20547-2 - Characterization of the metabolome of anti-phytopatogen microorganisms by Multidimensional Gas Chromatography and Simple / Tandem Mass Spectrometry (GC×GC-QMS and GC×GC-MS/MS)
• Grantee: Joao Raul Belinato de Souza
• Support type: Scholarships in Brazil - Doctorate
• FAPESP's process: 17/25490-1 - Synthesis and application of ionic liquids to unveil the chemistry of fermentative processes
• Grantee: Leandro Wang Hantao
• Support type: Regular Research Grants
• FAPESP's process: 15/05059-9 - Development and evaluation of Consumable-free planar microfluidic modulators for comprehensive two-dimensional gas chromatography
• Grantee: Leandro Wang Hantao
• Support type: Regular Research Grants
• FAPESP's process: 11/51896-9 - Application of comprehensive bidimensional gas chromatography to the search of biomarkers for pathogen resistance on vegetables
• Grantee: Fabio Augusto
• Support type: Regular Research Grants