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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Nanohybrid hydrogels designed for transbuccal anesthesia

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Autor(es):
de Morais Ribeiro, Ligia Nunes [1] ; Franz-Montan, Michelle [2] ; Breitkreitz, Marcia Cristina [3] ; Rodrigues da Silva, Gustavo Henrique [1] ; de Castro, Simone Ramos [1] ; Guilherme, Viviane Aparecida [1] ; de Araujo, Daniele Ribeiro [4] ; de Paula, Eneida [1]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Univ Estadual Campinas, Inst Biol, Dept Biochem & Tissue Biol, UNICAMP, Rua Monteiro Lobato 255, BR-13083862 Campinas, SP - Brazil
[2] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Physiol Sci, Sao Paulo - Brazil
[3] Univ Estadual Campinas, Inst Chem, Dept Analyt Chem, Campinas, SP - Brazil
[4] ABC Fed Univ, Human & Nat Sci Ctr, Sao Paulo - Brazil
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: INTERNATIONAL JOURNAL OF NANOMEDICINE; v. 13, p. 6453-6463, 2018.
Citações Web of Science: 3
Resumo

Background: Local anesthesia in dentistry is by far the most terrifying procedure for patients, causing treatment interruption. None of the commercially available topical formulations is effective in eliminating the pain and phobia associated to the needle insertion and injection. Materials and methods: In this work we prepared a nanostructured lipid-biopolymer hydrogel for the sustained delivery of lidocaine-prilocaine (LDC-PLC) for transbuccal pre-anesthesia. The lipid was composed of optimized nanostructured lipid carriers (NLC) loaded with 5% LDC-PLC (NLC/LDC-PLC). The biopolymer counterpart was selected among alginate, xanthan (XAN), and chitosan matrices. The XAN-NLC hydrogel presented the most uniform aspect and pseudoplastic rheological profile, as required for topical use; therefore, it was selected for subsequent analyses. Accelerated stability tests under critical conditions (40 degrees C; 75% relative humidity) were conducted for 6 months, in terms of drug content (mg/g), weight loss (%), and pH. Results: In vitro LDC-PLC release profile through Franz diffusion cells revealed a bimodal kinetics with a burst effect followed by the sustained release of both anesthetics, for 24 hours. Structural analyses (fourier transform infrared spectroscopy, differential scanning calorimetry and scanning electron microscopy) gave details on the molecular organization of the hybrid hydrogel, confirming the synergic interaction between the components. Safety and efficacy were evaluated through in vitro cell viability (3T3, HaCat, and VERO cells) and in vivo antinociceptive (tail-flick, in mice) tests, respectively. In comparison to a control hydrogel and the eutectic mixture of 5% LDC-PLC cream (EMLA (R)), the XAN-NLC/LDC-PLC hybrid hydrogel doubled and quadrupled the anesthetic effect (8 hours), respectively. Conclusion: Considering such exciting results, this multifaceted nanohybrid system is now ready to be further tested in clinical trials. (AU)

Processo FAPESP: 14/25372-0 - Desenvolvimento de carreadores lipídicos nanoestruturados para encapsulação de anestésicos locais processados em matrizes biopoliméricas para aplicação tópica em mucosa oral
Beneficiário:Lígia Nunes de Morais Ribeiro
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 14/14457-5 - Carreadores baseados em lipídios (SLN/NLC e lipossomas com gradiente iônico) como estratégia para aumentar a encapsulação e a potência de anestésicos locais
Beneficiário:Eneida de Paula
Linha de fomento: Auxílio à Pesquisa - Temático