Identification of pathogenic and nonpathogenic Leptospira species of Brazilian isolates by Matrix Assisted Laser Desorption/Ionization and Time Flight mass spectrometry

Autor(es)

Karcher, Daniel ^[1] ; Grenfell, Rafaella C. ^[1] ; Moreno, Andrea Micke ^[2] ; Moreno, Luisa Zanolli ^[2] ; Vasconcellos, Silvio Arruda ^[2] ; Heinemann, Marcos B. ^[2] ; de Almeida Junior, Joao N. ^[3] ; Juliano, Luiz ^[1] ; Juliano, Maria A. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Escola Paulista Med, Dept Biofis, Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Fac Vet & Zootecnia, Dept Med Vet Prevent & Saude Anim, Sao Paulo, SP - Brazil

^[3] Univ Sao Paulo, Inst Med Trop Sao Paulo, Lab Micol Med, Div Lab Cent, Hosp Clin, Fac Med, LIM 03, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Journal of Microbiology; v. 49, n. 4, p. 900-908, OCT-DEC 2018.
• Citações Web of Science: 0

Resumo

ABSTRACT Matrix Assisted Laser Desorption/Ionization and Time of Flight mass spectrometry (MALDI-TOF MS) is a powerful tool for the identification of bacteria through the detection and analysis of their proteins or fragments derived from ribosomes. Slight sequence variations in conserved ribosomal proteins distinguish microorganisms at the subspecies and strain levels. Characterization of Leptospira spp. by 16S RNA sequencing is costly and time-consuming, and recent studies have shown that closely related species (e.g., Leptospira interrogans and Leptospira kirschneri) may not be discriminated using this technology. Herein, we report an in-house Leptospira reference spectra database using Leptospira reference strains that were validated with a collection of well-identified Brazilian isolates kept in the Bacterial Zoonosis Laboratory at the Veterinary Preventive Medicine and Animal Health Department at Sao Paulo University. In addition, L. interrogans and L. kirschneri were differentiated using an in-depth mass spectrometry analysis with ClinProTools™ software. In conclusion, our in-house reference spectra database has the necessary accuracy to differentiate pathogenic and non-pathogenic species and to distinguish L. interrogans and L. kirschneri. (AU)

• Processo FAPESP: 13/17136-2 - Caracterização de estirpes de Leptospira isoladas no Brasil através de tipificação por eletroforese em campo pulsado (PFGE) e análise comparativa de genomas.
• Beneficiário: Luisa Zanolli Moreno
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 12/50191-4 - Síntese, estudo cinético e aplicações de substratos e inibidores de enzimas proteolíticas
• Beneficiário: Maria Aparecida Juliano
• Modalidade de apoio: Auxílio à Pesquisa - Temático