Oviplate: A Convenient and Space-Saving Method to Perform Individual Oviposition Assays in Aedes aegypti

Autor(es)

Ioshino, Rafaella Sayuri ^[1] ; Carvalho, Danilo Oliveira ^[1] ; Santos Marques, Isabel Cristina ^[1] ; Fernandes, Ediane Saraiva ^[1] ; Capurro, Margareth Lara ^[1] ; Costa-da-Silva, Andre Luis ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Ciencias Biomed, Dept Parasitol, Lab Mosquitos Geneticamente Modificados, BR-05508000 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: INSECTS; v. 9, n. 3 SEP 2018.
• Citações Web of Science: 0

Resumo

Aedes aegypti is the principal vector of the urban arboviruses and the blood ingestion is important to produce the eggs in this species. To analyze the egg production in Ae. aegypti, researchers frequently use small cages or Drosophila vials to collect eggs from gravid females. Although it is affordable, the setup is time- and space-consuming, mainly when many mosquitoes need to be individually analyzed. This study presents an easy, cheap, and space-saving method to perform individual oviposition assays in Ae. aegypti using cell culture plates. This new method to access fecundity rate was named oviplate. The oviplates are setup with 12- or 24-well plates, distilled water and filter paper and they are 78 to 88% cheaper than the traditional Drosophila vial assay, respectively. Furthermore, to allocate 72 vitellogenic females in an insectary using Drosophila vial is necessary 4100 cm(3) against 1400 cm(3) and 700 cm(3) when using 12- and 24-well plates, respectively. No statistical differences were found between the number of eggs laid in Drosophila vials and the oviplates, validating the method. The oviplate method is an affordable, and time- and space-efficient device, and it is simpler to perform individual fecundity analyses in Ae. aegypti. (AU)

• Processo FAPESP: 13/19921-9 - Avaliação e melhoramento de linhagens transgênicas de Aedes aegypti para controle de transmissão de dengue
• Beneficiário: Margareth de Lara Capurro-Guimarães
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/17766-9 - Abordagem sistêmica no estudo da permissividade do Anticarsia gemmatalis múltiplo nucleopoliedrovírus (AgMNPV)
• Beneficiário: Paolo Marinho de Andrade Zanotto
• Modalidade de apoio: Auxílio à Pesquisa - Regular