Polynitroxide copolymers to reduce biofilm fouling on surfaces

Autor(es)

Boase, Nathan R. B. ^[1] ; Torres, Marcelo D. T. ^{[2, 3, 4]} ; Fletcher, Nicholas L. ^{[5, 6]} ; de la Fuente-Nunez, Cesar ^{[2, 3]} ; Fairfull-Smith, Kathryn E. ^[1]

Afiliação do(s) autor(es)

^[1] QUT, Sch Chem Phys & Mech Engn, 2 George St, Brisbane, Qld 4000 - Australia

^[2] MIT, Dept Biol Engn & Elect Engn & Comp Sci, MIT Synthet Biol Ctr, Synthet Biol Grp, Res Lab Elect, 77 Massachusetts Ave, Cambridge, MA 02139 - USA

^[3] Broad Inst Massachusetts Inst Technol & Harvard, 415 Main St, Cambridge, MA - USA

^[4] Univ Fed ABC, Ctr Ciencias Nat & Humanas, 5001 Ave Estados, Santo Andre, SP - Brazil

^[5] Univ Queensland, Ctr Adv Imaging, St Lucia, Qld 4072 - Australia

^[6] Univ Queensland, Australian Inst Bioengn & Nanotechnol, ARC Ctr Excellence Convergent Bionano Sci & Techn, St Lucia, Qld 4072 - Australia

• Tipo de documento: Artigo Científico
• Fonte: Polymer Chemistry; v. 9, n. 43, p. 5308-5318, NOV 21 2018.
• Citações Web of Science: 5

Resumo

Biofilms are highly organised colonies of microorganisms, at a surface or an interface, which are extremely resistant to treatment with biocides or antimicrobials. Consequently, biofilms are the primary cause of fouling and persistent infections in both industrial and clinical settings. Thus, there is a clear need to develop coatings that are able to prevent the growth of biofilms on surfaces. Herein, we report the development of polynitroxides for use as anti-biofilm coatings. We demonstrate that we can tune the composition of the nitroxide 2,2,6,6-tetramethyl-4-piperidyl methacrylate in a copolymer with methyl methacrylate in order to control the surface concentration of nitroxides in the resulting thin films. The prepared films are able to reduce biofilm fouling by Pseudomonas aeruginosa (PAO1) at nitroxide monomer concentrations as low as 30 wt%. The nitroxide containing materials show no difference in toxicity to mammalian cells, compared to poly(methyl methacrylate), in a proliferation assay with 3T3 fibroblast cells. This is the first demonstration of surface tethered nitroxides with activity against biofilms and provides new opportunities for developing antifouling surface coatings. (AU)

• Processo FAPESP: 16/24413-0 - Peptídeos anfipáticos catiônicos antimicrobianos e antibiofilmes
• Beneficiário: Marcelo Der Torossian Torres
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado