Recent advances in LC-MS/MS methods to determine endocannabinoids in biological samples: Application in neurodegenerative diseases

Autor(es)

Marchioni, Camila ^[1] ; de Souza, Israel Donizeti ^[2] ; Acquaro Junior, Vinicius Ricardo ^[2] ; de Souza Crippa, Jose Alexandre ^[3] ; Tumas, Vitor ^[3] ; Costa Queiroz, Maria Eugenia ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Ave Bandeirantes 3900, BR-14040901 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Dept Quim, Ave Bandeirantes 3900, BR-14040901 Ribeirao Preto, SP - Brazil

^[3] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Neurosci & Behav, Sao Paulo - Brazil

• Tipo de documento: Artigo de Revisão
• Fonte: Analytica Chimica Acta; v. 1044, p. 12-28, DEC 31 2018.
• Citações Web of Science: 12

Resumo

Endocannabinoids (ECs) are endogenous lipid-based retrograde neurotransmitters that bind to cannabinoid receptors {[}cannabinoid receptor 1 (CB1) and cannabinoid receptor 2 (CB2)]. Many ECs have been characterized; anandamide (AEA) and 2-arachidonoyl glycerol (2-AG) are still considered the primary ECs signaling mediators. Dysregulation of ECs has been implicated in a wide range of pathologies, including neurodegenerative diseases. Understanding how ECs participate in neurological diseases is important to describe the pathology and to establish new treatments. Considering the physicochemical properties of ECs, liquid chromatography coupled to tandem mass spectrometry has become the reference method to determine these endogenous substances, in trace levels, in different biological samples. This review describes the recent advances in LC-MS/MS methods designed to determine ECs in complex biological matrixes. The advantages, limitations, selectivity, matrix effect, and sensitivity associated with each approach are emphasized. This article comprises three sections: (I) sample preparation techniques (conventional, microextraction, and online systems), (II) chromatographic methods (especially LC-MS/MS), and (III) relationship between ECs levels and neurodegenerative diseases. (C) 2018 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 17/02147-0 - Cromatografia líquida em uma gota e seu acoplamento com espectrometria de massas: estratégias instrumentais, desenvolvimento de materiais, automatização e aplicações analíticas
• Beneficiário: Fernando Mauro Lanças
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 15/07619-1 - Cromatografia líquida acoplada à espectrometria de massas em tandem no modo column switching para análises de endocanabinóides e de fármacos em amostras biológicas
• Beneficiário: Maria Eugênia Queiroz Nassur
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 16/13639-8 - Cromatografia líquida de ultra eficiência acoplada à espectrometria de massas em Tandem no modo column switching para a determinação de endocanabinoides e canabinoides em amostras biológicas de pacientes com a Doença de Parkinson
• Beneficiário: Camila Marchioni
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto