Resistance training in young men induces muscle transcriptome-wide changes associated with muscle structure and metabolism refining the response to exercise-induced stress

Autor(es):Mostrar menos -

Damas, Felipe ^{[1, 2]} ; Ugrinowitsch, Carlos ^[1] ; Libardi, Cleiton A. ^[2] ; Jannig, Paulo R. ^[1] ; Hector, Amy J. ^[3] ; McGlory, Chris ^[3] ; Lixandrao, Manoel E. ^[1] ; Vechin, Felipe C. ^[1] ; Montenegro, Horacio ^[4] ; Tricoli, Valmor ^[1] ; Roschel, Hamilton ^[1] ; Phillips, Stuart M. ^[3]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Phys Educ & Sport, Av Prof Mello Moraes 65, BR-05508030 Sao Paulo, SP - Brazil

^[2] Univ Fed Sao Carlos, Dept Phys Educ, MUSCULAB, Rod Washington Luiz, Km 235, SP310, BR-13565905 Sao Carlos, SP - Brazil

^[3] McMaster Univ, Dept Kinesiol, 1280 Main St West, Hamilton, ON L8S 4K1 - Canada

^[4] MB Bioinformat, Rua Jose Martins 603, BR-13084175 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: EUROPEAN JOURNAL OF APPLIED PHYSIOLOGY; v. 118, n. 12, p. 2607-2616, DEC 2018.
• Citações Web of Science: 2

Resumo

BackgroundGene expression is an important process underpinning the acute and chronic adaptive response to resistance exercise (RE) training.PurposeTo investigate the effect of training status on vastus lateralis muscle global transcriptome at rest and following acute RE.MethodsMuscle biopsies of nine young men (age: 26(2)years; body mass: 69(9)kg; height 172(6)cm) who undertook RE training for 10weeks were collected pre and 24h post-RE in the untrained (W1) and trained (W10) states and analysed using microarray. Tests of differential expression were conducted for rested and after RE contrasts in both training states. To control for false discovery rate (FDR), multiple testing correction was performed at a cut-off of FDR<0.05.ResultsUnaccustomed RE (at W1) upregulated muscle gene transcripts related to stress (e.g., heat shock proteins), damage and inflammation, structural remodelling, protein turnover and increased translational capacity. Trained muscles (at W10) showed changes in the transcriptome signature regarding the regulation of energy metabolism, favouring a more oxidative one, upregulated antioxidant- and immune-related genes/terms, and gene transcripts related to the cytoskeleton and extracellular matrix, muscle contraction, development and growth.ConclusionsThese results highlight that chronic repetition of RE changes muscle transcriptome response towards a more refined response to RE-induced stress. (AU)

• Processo FAPESP: 14/19594-0 - O papel do dano muscular na modulação da síntese protéica muscular
• Beneficiário: Felipe Romano Damas Nogueira
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 16/24259-1 - Individualidade biológica humana versus variáveis do treinamento de força: o que realmente importa para a hipertrofia muscular?
• Beneficiário: Felipe Romano Damas Nogueira
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 17/04299-1 - Individualidade biológica humana versus variáveis do treinamento de força: o que realmente importa para a hipertrofia muscular?
• Beneficiário: Cleiton Augusto Libardi
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 12/24499-1 - O papel da magnitude de dano muscular nas respostas hipertróficas
• Beneficiário: Felipe Romano Damas Nogueira
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 13/21218-4 - O papel da magnitude de dano muscular nas respostas hipertróficas
• Beneficiário: Cleiton Augusto Libardi
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 18/13064-0 - Respostas individuais da síntese de proteína muscular à modulação de variáveis de treinamento de força
• Beneficiário: Felipe Romano Damas Nogueira
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado