Resistance training in young men induces muscle transcriptome-wide changes associated with muscle structure and metabolism refining the response to exercise-induced stress

Author(s):Show less -

Damas, Felipe ^{[1, 2]} ; Ugrinowitsch, Carlos ^[1] ; Libardi, Cleiton A. ^[2] ; Jannig, Paulo R. ^[1] ; Hector, Amy J. ^[3] ; McGlory, Chris ^[3] ; Lixandrao, Manoel E. ^[1] ; Vechin, Felipe C. ^[1] ; Montenegro, Horacio ^[4] ; Tricoli, Valmor ^[1] ; Roschel, Hamilton ^[1] ; Phillips, Stuart M. ^[3]

Affiliation

^[1] Univ Sao Paulo, Sch Phys Educ & Sport, Av Prof Mello Moraes 65, BR-05508030 Sao Paulo, SP - Brazil

^[2] Univ Fed Sao Carlos, Dept Phys Educ, MUSCULAB, Rod Washington Luiz, Km 235, SP310, BR-13565905 Sao Carlos, SP - Brazil

^[3] McMaster Univ, Dept Kinesiol, 1280 Main St West, Hamilton, ON L8S 4K1 - Canada

^[4] MB Bioinformat, Rua Jose Martins 603, BR-13084175 Campinas, SP - Brazil

• Document type: Journal article
• Source: EUROPEAN JOURNAL OF APPLIED PHYSIOLOGY; v. 118, n. 12, p. 2607-2616, DEC 2018.
• Web of Science Citations: 2

Abstract

BackgroundGene expression is an important process underpinning the acute and chronic adaptive response to resistance exercise (RE) training.PurposeTo investigate the effect of training status on vastus lateralis muscle global transcriptome at rest and following acute RE.MethodsMuscle biopsies of nine young men (age: 26(2)years; body mass: 69(9)kg; height 172(6)cm) who undertook RE training for 10weeks were collected pre and 24h post-RE in the untrained (W1) and trained (W10) states and analysed using microarray. Tests of differential expression were conducted for rested and after RE contrasts in both training states. To control for false discovery rate (FDR), multiple testing correction was performed at a cut-off of FDR<0.05.ResultsUnaccustomed RE (at W1) upregulated muscle gene transcripts related to stress (e.g., heat shock proteins), damage and inflammation, structural remodelling, protein turnover and increased translational capacity. Trained muscles (at W10) showed changes in the transcriptome signature regarding the regulation of energy metabolism, favouring a more oxidative one, upregulated antioxidant- and immune-related genes/terms, and gene transcripts related to the cytoskeleton and extracellular matrix, muscle contraction, development and growth.ConclusionsThese results highlight that chronic repetition of RE changes muscle transcriptome response towards a more refined response to RE-induced stress. (AU)

• FAPESP's process: 14/19594-0 - The role of muscle damage in muscle protein synthesis modulation
• Grantee: Felipe Romano Damas Nogueira
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• FAPESP's process: 16/24259-1 - Human biological individuality versus resistance-training variables modulation: what really matters for muscle hypertrophy?
• Grantee: Felipe Romano Damas Nogueira
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 17/04299-1 - Human biological individuality versus resistance-training variables modulation: what really matters for muscle hypertrophy?
• Grantee: Cleiton Augusto Libardi
• Support Opportunities: Regular Research Grants
• FAPESP's process: 12/24499-1 - The role of the magnitude of muscle damage in hypertrophic responses
• Grantee: Felipe Romano Damas Nogueira
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 13/21218-4 - The role of the magnitude of muscle damage in hypertrophic responses
• Grantee: Cleiton Augusto Libardi
• Support Opportunities: Regular Research Grants
• FAPESP's process: 18/13064-0 - Individual muscle protein synthesis responses to the modulation of resistance training variables
• Grantee: Felipe Romano Damas Nogueira
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor