Genome-wide identification and expression analysis of the molecular chaperone binding protein BiP genes in Citrus

Autor(es)

Guimaraes, Roney Fontes ^[1] ; Camillo, Luciana Rodrigues ^[1] ; Sousa, Aurizangela Oliveira ^[1] ; Goncalves, Luana Pereira ^[1] ; Cardoso Macedo, Jamilly Costa ^[1] ; Siqueira, Giovano Sousa ^[1] ; Magalhaes Barbosa, Antonia Marlene ^[2] ; Alvim, Fatima Cerqueira ^[1] ; Cardoso Costa, Marcio Gilberto ^[1] ; Pirovani, Carlos Priminho ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Santa Cruz, Dept Ciencias Biol, Ctr Biotecnol & Genet, Rodovia Jorge Amado Km 16, BR-45662900 Ilheus, BA - Brazil

^[2] Univ Estadual Santa Cruz, Dept Ciencias Agr & Ambientais, Rodovia Jorge Amado Km 16, BR-45662900 Ilheus, BA - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Tree Genetics & Genomes; v. 14, n. 6 DEC 2018.
• Citações Web of Science: 0

Resumo

Here, we report for the first time the genome-wide identification and expression analysis of the molecular chaperone BiP genes in Citrus. Six genes encoding the conserved protein domain family GPR78/BiP/KAR2 were identified in the genome of Citrus sinensis and C. clementina. Two of them, named here as CsBiP1 and CsBiP2, were classified as true BiPs based on their deduced amino acid sequences. Alignment of the deduced amino acid sequences of CsBiP1 and CsBiP2 with BiP homologs from soybean and Arabidopsis showed that they contain all the conserved functional motifs of BiPs. Analysis of the promoter region of CsBiPs revealed the existence of cis-acting regulatory sequences involved in abiotic, heat-shock, and endoplasmic reticulum (ER) stress responses. Publicly available RNA-seq data indicated that CsBiP1 is abundantly expressed in leaf, flower, fruit, and callus, whereas CsBiP2 expression is rarely detected in any tissues under normal conditions. Comparative quantitative real-time PCR (qPCR) analysis of expression of these genes between C. sinensis grafted on the drought-tolerant Rangpur lime (C. limonia) and -sensitive Flying Dragon trifoliate orange (Poncirus trifoliata) rootstocks showed that CsBiP1 was upregulated by drought stress on the former but downregulated on the latter, whereas the CsBiP2 mRNA levels were downregulated on drought-stressed Flying Dragon, but remained constant on Rangpur. CsBiP2 upregulation was only observed in C. sinensis seedlings subjected to osmotic and cold treatments. Taken together, these results indicate the existence of two highly conserved BiP genes in Citrus that are differentially regulated in the different tissues and in response to abiotic stresses. (AU)

• Processo FAPESP: 14/50880-0 - INCT 2014: de genômica comparativa e funcional e melhoramento assistido de citros
• Beneficiário: Marcos Antonio Machado
• Modalidade de apoio: Auxílio à Pesquisa - Temático