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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Electrospun nanofiber blend with improved mechanical and biological performance

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Autor(es):
Lobo, Anderson Oliveira [1, 2, 3, 4] ; Afewerki, Samson [2, 4] ; Machado de Paula, Mirian Michele [5] ; Ghannadian, Paria [5] ; Marciano, Fernanda Roberta [5, 3] ; Zhang, Yu Shrike [2, 4] ; Webster, Thomas Jay [5] ; Khademhosseini, Ali [2, 4, 6, 7, 8, 9, 10]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] UFPI Fed Univ Piaui, LIMAv Interdisciplinary Lab Adv Mat, PPGCM Mat Sci & Engn Grad Program, BR-64049550 Teresina, Piaui - Brazil
[2] MIT, Harvard MIT Div Hlth Sci & Technol, 77 Massachusetts Ave, Cambridge, MA 02139 - USA
[3] Brasil Univ, Inst Sci & Technol, BR-08230030 Sao Paulo - Brazil
[4] Harvard Med Sch, Brigham & Womens Hosp, Div Engn Med, Dept Med, Cambridge, MA 02139 - USA
[5] Northeastern Univ, Dept Chem Engn, Boston, MA 02115 - USA
[6] Univ Calif Los Angeles, Dept Bioengn, Dept Chem & Biomol Engn, Henry Samueli Sch Engn & Appl Sci, Los Angeles, CA 90095 - USA
[7] Univ Calif Los Angeles, David Geffen Sch Med, Dept Radiol, Los Angeles, CA 90095 - USA
[8] Univ Calif Los Angeles, Ctr Minimally Invas Therapeut, Los Angeles, CA 90095 - USA
[9] Univ Calif Los Angeles, Calif NanoSyst Inst CNSI, Los Angeles, CA 90095 - USA
[10] Konkuk Univ, Dept Bioind Technol, Seoul 143701 - South Korea
Número total de Afiliações: 10
Tipo de documento: Artigo Científico
Fonte: INTERNATIONAL JOURNAL OF NANOMEDICINE; v. 13, p. 7891-7903, 2018.
Citações Web of Science: 3
Resumo

Background: Here, electrospun fibers based on a blend of polycaprolactone (PCL), poly(ethylene glycol) (PEG), and gelatin methacryloyl (GelMA) were developed. The careful choice of this polymer combination allowed for the preparation of a biomaterial that preserved the mechanical strength of PCL, while at the same time improving the hydrophilicity of the blended material and human osteoblast maturation. Methods: The morphology, chemical structure, wettability, and mechanical properties before and after UV photocrosslinking were evaluated. Furthermore, human osteoblasts (hFOB) were cultivated for up to 21 days on the scaffolds, and their potential to upregulate cell proliferation, alkaline phosphatase (ALP) activity, and calcium deposition were investigated. Results: Contact angle measurement results showed that the developed scaffolds presented hydrophilic properties after PEG and GelMA incorporation before (25 degrees) and after UV photocrosslinking (69 degrees) compared to pure PCL (149 degrees). PCL: PEG: GelMA-UV displayed a slight increase in mechanical strength (elastic modulus similar to 37 MPa) over PCL alone (similar to 33 MPa). Normally, an increase in strength of fibers leads to a decrease in elongation at break, due to the material becoming less deformable and stiffer, thus leading to breaks at low strain. This behavior was observed by comparing PCL (elongation at break similar to 106%) and PCL:PEG:GelMA-UV (similar to 50%). Moreover, increases in ALP activity (10-fold at day 14) and calcium deposition (1.3-fold at day 21) by hFOBs were detected after PEG and GelMA incorporation after UV photocrosslinking compared to pure PCL. Ultrathin and hydrophilic fibers were obtained after PEG and GelMA incorporation after UV photocrosslinking, but the strength of PCL was maintained. Interestingly, those ultrathin fiber characteristics improved hFOB functions. Conclusion: These findings appear promising for the use of these electrospun scaffolds, based on the combination of polymers used here for numerous orthopedic applications. (AU)

Processo FAPESP: 15/09697-0 - Eletrofiação de novos nanobiomateriais funcionais à base de peptídeos e polímeros bottlebrush para engenharia tecidual
Beneficiário:Anderson de Oliveira Lobo
Linha de fomento: Bolsas no Exterior - Pesquisa
Processo FAPESP: 16/00575-1 - Biomembranas funcionais a base de peptídeos e polímeros bottlebrush aplicados a engenharia de tecidos
Beneficiário:Fernanda Roberta Marciano
Linha de fomento: Bolsas no Exterior - Pesquisa