LPS-induced alterations in reproductive organs and liver pigmentation in the toad Rhinella diptycha (Bufonidae)

Autor(es)

Gregorio, L. S. ^{[1, 2]} ; Freitas, J. S. ^[3] ; Franco-Belussi, L. ^[4] ; De Oliveira, C. ^[4]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ, UNESP, Dept Biol, Grad Program Anim Biol, Sao Jose Do Rio Preto, SP - Brazil

^[2] Rua Cristovao Colombo, 2264 Jardim Nazareth, BR-15054000 Sao Jose Do Rio Preto, SP - Brazil

^[3] Univ Sao Paulo, Sch Engn Sao Carlos, Ctr Ecotoxicol & Appl Ecol, Dept Hydraul & Sanitat, Sao Carlos, SP - Brazil

^[4] Sao Paulo State Univ, UNESP, Dept Biol, Sao Jose Do Rio Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: CANADIAN JOURNAL OF ZOOLOGY; v. 96, n. 12, p. 1336-1345, DEC 2018.
• Citações Web of Science: 0

Resumo

Anurans can be naturally infected by the bacterium Escherichia coli (Migula, 1895) Castellani and Chalmers, 1919, which contain lipopolysaccharides (LPS) in their external membrane. LPS affects reproductive functions in mammals and extracutaneous pigmentary system of anurans. Besides the functional gonad, bufonid amphibians have an undeveloped female organ (Bidder's organ), whose function is poorly known. We aimed to investigate the effects of LPS on testis and Bidder's organ germ cells and on liver pigmentation in the toad Rhinella diptycha (Cope, 1862). Animals were inoculated with 0.1 mL of saline solution (control) or LPS solution (18 mg/kg) and then euthanized after 24 or 48 h. The organs were processed for histology and then evaluated with morphometric analysis. The LPS decreased the area of bundle spermatozoa and increased lumen spermatozoa, while in Bidder's organ, the number of degenerating oocytes decreased. Liver melanin increased after 24 h and decreased after 48 h. Our results suggest that LPS delays the release of spermatozoa, possibly due to alterations in serum levels of testosterone and (or) gonadotropins, which may also explain fewer degrading cells in Bidder's organ. Increased melanin in the liver was expected due to the bactericidal role of this pigment. Thus, LPS affected reproductive organs and induced cytoprotective responses in the liver of amphibians. (AU)

• Processo FAPESP: 14/00946-4 - Pigmentação visceral em animais ectotérmicos: imunidade inata e defesa contra UV
• Beneficiário: Lilian Franco Belussi
• Linha de fomento: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 11/21519-9 - Ação do LPS de Escherichia coli no órgão de Bidder, testículo e fígado de machos Rhinella schneideri (Anura: Bufonidae)
• Beneficiário: Lara Salgueiro de Gregorio
• Linha de fomento: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 13/02067-5 - Efeitos tóxicos dos herbicidas Atrazina e Roundup em Eupemphix nattereri (Leiuperidae) e Rhinella schneideri (Bufonidae): alterações morfológicas sob diferentes concentrações e condições abióticas (pH, temperatura e fotoperíodo)
• Beneficiário: Classius de Oliveira
• Linha de fomento: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/16948-3 - Influência da temperatura na toxicidade dos herbicidas Boral 500SC® e GAMIT em larvas de anuros Eupemphix nattereri (Leptodactylidae) e Rhinella schneideri (Bufonidae)
• Beneficiário: Juliane Silberschmidt Freitas
• Linha de fomento: Bolsas no Brasil - Doutorado