Differential regulation of cysteine oxidative post-translational modifications in high and low aerobic capacity

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Souza, Rodrigo W. A. ^[1] ; Alves, Christiano R. R. ^{[1, 2]} ; Medeiros, Alessandra ^[3] ; Rolim, Natale ^[4] ; Silva, Gustavo J. J. ^[4] ; Moreira, Jose B. N. ^[4] ; Alves, Marcia N. ^[4] ; Wohlwend, Martin ^[4] ; Gebriel, Mohammed ^{[5, 6, 7]} ; Hagen, Lars ^{[5, 6, 7]} ; Sharma, Animesh ^{[5, 6, 7]} ; Koch, Lauren G. ^[8] ; Britton, Steven L. ^{[9, 10]} ; Slupphaug, Geir ^{[5, 6, 7]} ; Wisloff, Ulrik ^{[11, 4]} ; Brum, Patricia C. ^[1]

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^[1] Univ Sao Paulo, Sch Phys Educ & Sport, Sao Paulo - Brazil

^[2] Harvard Med Sch, Sect Integrat Physiol & Metab, Joslin Diabet Ctr, Boston, MA - USA

^[3] Univ Fed Sao Paulo, Biosci Dept, Santos - Brazil

^[4] Norwegian Univ Sci & Technol NTNU, Dept Circulat & Med Imaging, KG Jebsen Ctr Exercise Med, Trondheim - Norway

^[5] Norwegian Univ Sci & Technol NTNU, Dept Canc Res & Mol Med, Trondheim - Norway

^[6] Norwegian Univ Sci & Technol NTNU, PROMEC Core Facil Prote & Mod, Trondheim - Norway

^[7] Cent Norway Reg Hlth Author, Trondheim - Norway

^[8] Univ Toledo, Dept Physiol & Pharmacol, 2801 W Bancroft St, Toledo, OH 43606 - USA

^[9] Univ Michigan, Dept Mol & Integrat Physiol, Ann Arbor, MI 48109 - USA

^[10] Univ Michigan, Dept Anesthesiol, Sch Med, Ann Arbor, MI 48109 - USA

^[11] Univ Queensland, Sch Human Movement & Nutr Sci, Brisbane, Qld - Australia

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 8, DEC 11 2018.
• Citações Web of Science: 1

Resumo

Given the association between high aerobic capacity and the prevention of metabolic diseases, elucidating the mechanisms by which high aerobic capacity regulates whole-body metabolic homeostasis is a major research challenge. Oxidative post-translational modifications (Ox-PTMs) of proteins can regulate cellular homeostasis in skeletal and cardiac muscles, but the relationship between Ox-PTMs and intrinsic components of oxidative energy metabolism is still unclear. Here, we evaluated the Ox-PTM profile in cardiac and skeletal muscles of rats bred for low (LCR) and high (HCR) intrinsic aerobic capacity. Redox proteomics screening revealed different cysteine (Cys) Ox-PTM profile between HCR and LCR rats. HCR showed a higher number of oxidized Cys residues in skeletal muscle compared to LCR, while the opposite was observed in the heart. Most proteins with differentially oxidized Cys residues in the skeletal muscle are important regulators of oxidative metabolism. The most oxidized protein in the skeletal muscle of HCR rats was malate dehydrogenase (MDH1). HCR showed higher MDH1 activity compared to LCR in skeletal, but not cardiac muscle. These novel findings indicate a clear association between Cys Ox-PTMs and aerobic capacity, leading to novel insights into the role of Ox-PTMs as an essential signal to maintain metabolic homeostasis. (AU)

• Processo FAPESP: 14/25957-9 - Influência da aptidão aeróbica na homeostase redox: identificação e implicação biológica da oxidação de resíduos cisteína em proteínas do músculo cardíaco e esquelético
• Beneficiário: Rodrigo Wagner Alves de Souza
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 12/25240-1 - Efeitos do treinamento físico aeróbio intervalado em alta intensidade no músculo esquelético de ratos inoculados com o tumor Walker 256
• Beneficiário: Christiano Robles Rodrigues Alves
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Mestrado
• Processo FAPESP: 14/03016-8 - Caquexia do câncer: efeitos do treinamento físico aeróbio sobre o músculo esquelético em modelo animal
• Beneficiário: Christiano Robles Rodrigues Alves
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 15/22814-5 - Câncer e coração: novos paradigmas de diagnóstico e tratamento
• Beneficiário: Carlos Eduardo Negrão
• Modalidade de apoio: Auxílio à Pesquisa - Temático