Characterizing the embryonic development of B. hygida (Diptera: Sciaridae) following enzymatic treatment to permeabilize the serosal cuticle

Autor(es)

Cardoso Uliana, Joao Vitor ^[1] ; Pereira Brancini, Guilherme Thomaz ^[1] ; Castelli-Gair Hombria, James ^[2] ; Digiampietri, Luciano Antonio ^[3] ; Andrioli, Luiz Paulo ^[3] ; Monesi, Nadia ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Ave Cafe, BR-14040903 Ribeirao Preto, SP - Brazil

^[2] CSIC JA UPO, Ctr Andaluz Biol Desarrollo, Carretera Utrera Km1, Seville 41013 - Spain

^[3] Univ Sao Paulo, Escola Artes Ciencias & Humanidades, R Arlindo Bettio 1000, BR-03828000 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: MECHANISMS OF DEVELOPMENT; v. 154, p. 270-276, DEC 2018.
• Citações Web of Science: 0

Resumo

Understanding the evolution of the developmental programs active during dipteran embryogenesis depends on comparative studies. As a counterpoint to the intensively investigated and highly derived cyclorrhaphan flies that include the model organism Drosophila melanogaster, we are studying the basal Diptera Bradysia hygida, a member of the Sciaridae family that is amenable to laboratory cultivation. Here we describe the B. hygida embryogenesis, which lasts 9 days at 22 degrees C. The use of standard fixation D. melanogaster protocols resulted in embryos refractory to DAPI staining and to overcome this, a new enzyme-based method was developed. Calcofluor-White staining of enzimatically-treated embryos revealed that this method removes chitin from the serosal cuticle surrounding the B. hygida embryo. Chitin is one of the main components of serosal cuticles and searches in a B. hygida embryonic transcriptome database revealed conservation of the chitin synthesis pathway, further supporting the occurrence of chitin biosynthesis in B. hygida embryos. Combining the enzymatic treatment protocol with the use of both DIC and fluorescence microscopy allowed the first complete description of the B. hygida embryogenesis. Our results constitute an important step towards the understanding of early development of a basal Diptera and pave the way for future evo-devo studies. (AU)

• Processo FAPESP: 16/25325-8 - As glândulas protorácicas de dípteros: estudos morfológicos, genômicos e funcionais nos dípteros B. hygida e D. melanogaster
• Beneficiário: Nadia Monesi
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 16/00412-5 - Caracterização do perfil de expressão do RNAm BhC4-1-LacZ em embriões de linhagens das séries (-824/-187), (-488/-187) e (-850/+40) de D. melanogaster
• Beneficiário: João Vitor Cardoso Uliana
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 15/24305-0 - Avaliação dos efeitos da luz azul e da luz vermelha na tolerância ao estresse e no proteoma do fungo entomopatogênico Metarhizium acridum
• Beneficiário: Guilherme Thomaz Pereira Brancini
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 14/14318-5 - Interações gênicas na região anterior da blastoderme sincicial de Drosophila melanogaster e do sciarídeo Trichosia pubescens
• Beneficiário: Luiz Paulo Moura Andrioli
• Modalidade de apoio: Auxílio à Pesquisa - Regular