| Full text | |
| Author(s): |
Cardoso Uliana, Joao Vitor
[1]
;
Pereira Brancini, Guilherme Thomaz
[1]
;
Castelli-Gair Hombria, James
[2]
;
Digiampietri, Luciano Antonio
[3]
;
Andrioli, Luiz Paulo
[3]
;
Monesi, Nadia
[1]
Total Authors: 6
|
| Affiliation: | [1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Ave Cafe, BR-14040903 Ribeirao Preto, SP - Brazil
[2] CSIC JA UPO, Ctr Andaluz Biol Desarrollo, Carretera Utrera Km1, Seville 41013 - Spain
[3] Univ Sao Paulo, Escola Artes Ciencias & Humanidades, R Arlindo Bettio 1000, BR-03828000 Sao Paulo, SP - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | MECHANISMS OF DEVELOPMENT; v. 154, p. 270-276, DEC 2018. |
| Web of Science Citations: | 0 |
| Abstract | |
Understanding the evolution of the developmental programs active during dipteran embryogenesis depends on comparative studies. As a counterpoint to the intensively investigated and highly derived cyclorrhaphan flies that include the model organism Drosophila melanogaster, we are studying the basal Diptera Bradysia hygida, a member of the Sciaridae family that is amenable to laboratory cultivation. Here we describe the B. hygida embryogenesis, which lasts 9 days at 22 degrees C. The use of standard fixation D. melanogaster protocols resulted in embryos refractory to DAPI staining and to overcome this, a new enzyme-based method was developed. Calcofluor-White staining of enzimatically-treated embryos revealed that this method removes chitin from the serosal cuticle surrounding the B. hygida embryo. Chitin is one of the main components of serosal cuticles and searches in a B. hygida embryonic transcriptome database revealed conservation of the chitin synthesis pathway, further supporting the occurrence of chitin biosynthesis in B. hygida embryos. Combining the enzymatic treatment protocol with the use of both DIC and fluorescence microscopy allowed the first complete description of the B. hygida embryogenesis. Our results constitute an important step towards the understanding of early development of a basal Diptera and pave the way for future evo-devo studies. (AU) | |
| FAPESP's process: | 16/25325-8 - The dipteran protoracic glands: morphological, genomic and functional studies in the dipteran B. hygida and D. melanogaster |
| Grantee: | Nadia Monesi |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 16/00412-5 - Characterization of the BhC4-1-lacZ mRNA pattern of expression in embryos of the (-824/-187), (-488/-187) and (-850/+40) series in D. melanogaster transgenic lines |
| Grantee: | João Vitor Cardoso Uliana |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |
| FAPESP's process: | 15/24305-0 - Evaluating the effects of blue and red light on the stress tolerance and the proteome of the entomopathogenic fungus Metarhizium acridum |
| Grantee: | Guilherme Thomaz Pereira Brancini |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 14/14318-5 - Gene interactions in the anterior region of the syncytial blastoderm of Drosophila melanogaster and of the sciarid fly Trichosia pubescens |
| Grantee: | Luiz Paulo Moura Andrioli |
| Support Opportunities: | Regular Research Grants |