Inhibitory effect of probiotic Lactobacillus supernatants from the oral cavity on Streptococcus mutans biofilms

Autor(es)

Rossoni, Rodnei Dennis ^[1] ; Velloso, Marisol dos Santos ^[1] ; de Barros, Patricia Pimentel ^[1] ; de Alvarenga, Janaina Araujo ^[1] ; dos Santos, Jessica Diane ^[1] ; Chipoletti dos Santos Prado, Ana Carolina ^[1] ; Ribeiro, Felipe de Camargo ^[1] ; Anbinder, Ana Lia ^[1] ; Junqueira, Juliana Campos ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Inst Sci & Technol, Dept Biosci & Oral Diag, Sao Jose Dos Campos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Microbial Pathogenesis; v. 123, p. 361-367, OCT 2018.
• Citações Web of Science: 3

Resumo

Probiotics can release bioactive substances that can inhibit the growth and biofilm formation of pathogenic microorganisms such as Streptococcus mutans. In this context, we evaluated whether the supernatants of Lactobacillus strains isolated from caries-free subjects can inhibit S. mutans, one of the most important bacteria for dental caries. First, the supernatants of 22 Lactobacillus strains were screened for antibacterial activity against S. mutans in planktonic cultures. All 22 Lactobacillus strains studied (100%) showed antibacterial activity. Thereafter, the Lactobacillus strains with the greatest reductions in the planktonic S. mutans cultures were tested on biofilms. The L. fermentum 20.4, L. paracasei 11.6, L. paracasei 20.3 and L. paracasei 25.4 strains could significantly reduce the number of S. mutans cells in biofilms formed in hydroxyapatite (p < 0.05). This reduction was also confirmed by scanning electron microscopy analysis and was not caused by the decreased pH value in the medium (p > 0.05). In addition, the supernatants of these probiotic strains could also reduce the total biomass of S. mutans biofilms (p < 0.05). In conclusion, most of the Lactobacillus strains tested have some antibacterial activity against S. mutans. L. fermentum 20.4, L. paracasei 11.6, L. paracasei 20.3 and L. paracasei 25.4 produce bioactive substances that caused a significant reduction in S. mutans biofilms. (AU)

• Processo FAPESP: 14/11841-9 - Efeitos de Lactobacillus spp. isolados da cavidade bucal de indivíduos livres de cáries sobre Streptococcus mutans: estudo da ação probiótica
• Beneficiário: Marisol dos Santos Velloso
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 13/25181-8 - Isolamento e caracterização de Lactobacillus spp. da cavidade bucal e sua ação probiótica sob Candida albicans: formação de biofilme, infecção em modelos de invertebrados e expressão dos genes EFG1, HWP1 e ALS1
• Beneficiário: Rodnei Dennis Rossoni
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 17/19219-3 - Identificação de substâncias bioativas produzidas por Lactobacillus paracasei 28.4 para o controle da candidose bucal
• Beneficiário: Rodnei Dennis Rossoni
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 15/09770-9 - Isolamento e caracterização de Lactobacillus spp. da cavidade bucal e sua ação probiótica sob Candida albicans e Streptococcus mutans
• Beneficiário: Juliana Campos Junqueira
• Modalidade de apoio: Auxílio à Pesquisa - Regular