Screening method for simultaneous detection of elaidic and vaccenic trans fatty acid isomers by capillary zone electrophoresis

Autor(es)

Amorim, Tatiane Lima ^[1] ; Duarte, Lucas Mattos ^[1] ; Dos Santos, Helio F. ^[2] ; Leal de Oliveira, Marcone Augusto ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Juiz de Fora, Dept Chem, Inst Exact Sci, GQAQ, BR-36036330 Juiz De Fora, MG - Brazil

^[2] Univ Fed Juiz de Fora, Dept Chem, Inst Exact Sci, NEQC, BR-36036330 Juiz De Fora, MG - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Analytica Chimica Acta; v. 1048, p. 212-220, FEB 7 2019.
• Citações Web of Science: 4

Resumo

A novel screening method for the simultaneous detection of elaidic and vaccenic trans fatty acid isomers by CZE-UV was proposed and applied to brazilian spreadable cheese analysis. Optimized background electrolyte was composed by 10 mmol L-1 heptakis (2, 3, 6-tri-o-methyl)-beta cyclodextrin, 24 mmol L-1 sodium tetraborate buffer solution, 45% methanol and 15% acetonitrile. Sample preparation comprised only a saponification reaction and dilution of sample solution before analysis. The method proved to be simple, environmental friendly and sensitive (LOD calculated for elaidic and vaccenic acid were 0.0163 mmol L-1 and 0.0169 mmol L-1, respectively) and may be used to detect adulteration in dairy products, where the addition of hydrogenated vegetable fat to food is prohibited. (C) 2018 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 14/50867-3 - INCT 2014: Instituto Nacional de Ciência e Tecnologia de Bioanalítica
• Beneficiário: Marco Aurelio Zezzi Arruda
• Modalidade de apoio: Auxílio à Pesquisa - Temático