Hormonal control and target genes of ftz-f1 expression in the honeybee Apis mellifera: a positive loop linking juvenile hormone, ftz-f1, and vitellogenin

Autor(es)

Mello, T. R. P. ^[1] ; Aleixo, A. C. ^[1] ; Pinheiro, D. G. ^[2] ; Nunes, F. M. F. ^[3] ; Cristino, A. S. ^[4] ; Bitondi, M. M. G. ^[5] ; Barchuk, A. R. ^[6] ; Simoes, Z. L. P. ^[5]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Genet, Ribeirao Preto, SP - Brazil

^[2] Univ Estadual Paulista, UNESP, Fac Ciencias Agr & Vet, Jaboticabal, SP - Brazil

^[3] Univ Fed Sao Carlos, Ctr Ciencias Biol & Saude, Dept Genet & Evolucao, Sao Carlos, SP - Brazil

^[4] Univ Queensland, Translat Res Inst, Diamantina Inst, Brisbane, Qld - Australia

^[5] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Biol, Ribeirao Preto, SP - Brazil

^[6] Univ Fed Alfenas, UNIFAL MG, Inst Ciencias Biomed, Dept Biol Celular & Desenvolvimento, Alfenas, MG - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Insect Molecular Biology; v. 28, n. 1, p. 145-159, FEB 2019.
• Citações Web of Science: 0

Resumo

Ftz-f1 is an orphan member of the nuclear hormone receptor superfamily. A 20-hydroxyecdysone pulse allows ftz-f1 gene expression, which then regulates the activity of downstream genes involved in major developmental progression events. In honeybees, the expression of genes like vitellogenin (vg), prophenoloxidase and juvenile hormone-esterase during late pharate-adult development is known to be hormonally controlled in both queens and workers by increasing juvenile hormone (JH) titres in the presence of declining levels of ecdysteroids. Since Ftz-f1 is known for mediating intracellular JH signalling, we hypothesized that ftz-f1 could mediate JH action during the pharate-adult development of honeybees, thus controlling the expression of these genes. Here, we show that ftz-f1 has caste-specific transcription profiles during this developmental period, with a peak coinciding with the increase in JH titre, and that its expression is upregulated by JH and downregulated by ecdysteroids. RNAi-mediated knock down of ftz-f1 showed that the expression of genes essential for adult development (e.g. vg and cuticular genes) depends on ftz-f1 expression. Finally, a double-repressor hypothesis-inspired vg gene knock-down experiment suggests the existence of a positive molecular loop between JH, ftz-f1 and vg. (AU)

• Processo FAPESP: 05/03926-5 - Genômica funcional de Apis mellifera: busca de novos genes e redes funcionais no contexto do desenvolvimento, da diferenciação de castas e da reprodução
• Beneficiário: Zilá Luz Paulino Simões
• Linha de fomento: Auxílio à Pesquisa - Temático
• Processo FAPESP: 08/10757-3 - O fator de transcrição FTZ-f1 e o controle da expressão de vitelogenina em Apis mellifera
• Beneficiário: Tathyana Rachel Palo Mello
• Linha de fomento: Bolsas no Brasil - Doutorado
• Processo FAPESP: 11/03171-5 - Análise causal do desenvolvimento de Apis mellifera - genes reguladores e redes hierárquicas de expressão gênica na especificação de tecidos e órgãos
• Beneficiário: Zilá Luz Paulino Simões
• Linha de fomento: Auxílio à Pesquisa - Temático