AMPK signaling pathway: potential regulated targets and effects on gene expression...
| Full text | |
| Author(s): |
Mello, T. R. P.
[1]
;
Aleixo, A. C.
[1]
;
Pinheiro, D. G.
[2]
;
Nunes, F. M. F.
[3]
;
Cristino, A. S.
[4]
;
Bitondi, M. M. G.
[5]
;
Barchuk, A. R.
[6]
;
Simoes, Z. L. P.
[5]
Total Authors: 8
|
| Affiliation: | [1] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Genet, Ribeirao Preto, SP - Brazil
[2] Univ Estadual Paulista, UNESP, Fac Ciencias Agr & Vet, Jaboticabal, SP - Brazil
[3] Univ Fed Sao Carlos, Ctr Ciencias Biol & Saude, Dept Genet & Evolucao, Sao Carlos, SP - Brazil
[4] Univ Queensland, Translat Res Inst, Diamantina Inst, Brisbane, Qld - Australia
[5] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Biol, Ribeirao Preto, SP - Brazil
[6] Univ Fed Alfenas, UNIFAL MG, Inst Ciencias Biomed, Dept Biol Celular & Desenvolvimento, Alfenas, MG - Brazil
Total Affiliations: 6
|
| Document type: | Journal article |
| Source: | Insect Molecular Biology; v. 28, n. 1, p. 145-159, FEB 2019. |
| Web of Science Citations: | 0 |
| Abstract | |
Ftz-f1 is an orphan member of the nuclear hormone receptor superfamily. A 20-hydroxyecdysone pulse allows ftz-f1 gene expression, which then regulates the activity of downstream genes involved in major developmental progression events. In honeybees, the expression of genes like vitellogenin (vg), prophenoloxidase and juvenile hormone-esterase during late pharate-adult development is known to be hormonally controlled in both queens and workers by increasing juvenile hormone (JH) titres in the presence of declining levels of ecdysteroids. Since Ftz-f1 is known for mediating intracellular JH signalling, we hypothesized that ftz-f1 could mediate JH action during the pharate-adult development of honeybees, thus controlling the expression of these genes. Here, we show that ftz-f1 has caste-specific transcription profiles during this developmental period, with a peak coinciding with the increase in JH titre, and that its expression is upregulated by JH and downregulated by ecdysteroids. RNAi-mediated knock down of ftz-f1 showed that the expression of genes essential for adult development (e.g. vg and cuticular genes) depends on ftz-f1 expression. Finally, a double-repressor hypothesis-inspired vg gene knock-down experiment suggests the existence of a positive molecular loop between JH, ftz-f1 and vg. (AU) | |
| FAPESP's process: | 05/03926-5 - Functional genomics of Apis mellifera: search for new genes and functional networks in the context of development, different castes and reproduction |
| Grantee: | Zilá Luz Paulino Simões |
| Support type: | Research Projects - Thematic Grants |
| FAPESP's process: | 08/10757-3 - The transcription factor FTZ-f1 and the control os vitellogenin expression in Apis mellifera |
| Grantee: | Tathyana Rachel Palo Mello |
| Support type: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 11/03171-5 - Causal analysis of Apis mellifera development - regulatory genes and hierachical networks of gene expression in the specification of tissue and organs |
| Grantee: | Zilá Luz Paulino Simões |
| Support type: | Research Projects - Thematic Grants |