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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Hormonal control and target genes of ftz-f1 expression in the honeybee Apis mellifera: a positive loop linking juvenile hormone, ftz-f1, and vitellogenin

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Author(s):
Mello, T. R. P. [1] ; Aleixo, A. C. [1] ; Pinheiro, D. G. [2] ; Nunes, F. M. F. [3] ; Cristino, A. S. [4] ; Bitondi, M. M. G. [5] ; Barchuk, A. R. [6] ; Simoes, Z. L. P. [5]
Total Authors: 8
Affiliation:
[1] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Genet, Ribeirao Preto, SP - Brazil
[2] Univ Estadual Paulista, UNESP, Fac Ciencias Agr & Vet, Jaboticabal, SP - Brazil
[3] Univ Fed Sao Carlos, Ctr Ciencias Biol & Saude, Dept Genet & Evolucao, Sao Carlos, SP - Brazil
[4] Univ Queensland, Translat Res Inst, Diamantina Inst, Brisbane, Qld - Australia
[5] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Biol, Ribeirao Preto, SP - Brazil
[6] Univ Fed Alfenas, UNIFAL MG, Inst Ciencias Biomed, Dept Biol Celular & Desenvolvimento, Alfenas, MG - Brazil
Total Affiliations: 6
Document type: Journal article
Source: Insect Molecular Biology; v. 28, n. 1, p. 145-159, FEB 2019.
Web of Science Citations: 0
Abstract

Ftz-f1 is an orphan member of the nuclear hormone receptor superfamily. A 20-hydroxyecdysone pulse allows ftz-f1 gene expression, which then regulates the activity of downstream genes involved in major developmental progression events. In honeybees, the expression of genes like vitellogenin (vg), prophenoloxidase and juvenile hormone-esterase during late pharate-adult development is known to be hormonally controlled in both queens and workers by increasing juvenile hormone (JH) titres in the presence of declining levels of ecdysteroids. Since Ftz-f1 is known for mediating intracellular JH signalling, we hypothesized that ftz-f1 could mediate JH action during the pharate-adult development of honeybees, thus controlling the expression of these genes. Here, we show that ftz-f1 has caste-specific transcription profiles during this developmental period, with a peak coinciding with the increase in JH titre, and that its expression is upregulated by JH and downregulated by ecdysteroids. RNAi-mediated knock down of ftz-f1 showed that the expression of genes essential for adult development (e.g. vg and cuticular genes) depends on ftz-f1 expression. Finally, a double-repressor hypothesis-inspired vg gene knock-down experiment suggests the existence of a positive molecular loop between JH, ftz-f1 and vg. (AU)

FAPESP's process: 05/03926-5 - Functional genomics of Apis mellifera: search for new genes and functional networks in the context of development, different castes and reproduction
Grantee:Zilá Luz Paulino Simões
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 08/10757-3 - The transcription factor FTZ-f1 and the control os vitellogenin expression in Apis mellifera
Grantee:Tathyana Rachel Palo Mello
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 11/03171-5 - Causal analysis of Apis mellifera development: regulatory genes and hierachical networks of gene expression in the specification of tissue and organs
Grantee:Zilá Luz Paulino Simões
Support Opportunities: Research Projects - Thematic Grants