Actin from the apicomplexan Neospora caninum (NcACT) has different isoforms in 2D electrophoresis

Autor(es)

Baronil, Luciana ^[1] ; Pollo-Oliveira, Leticia ^[1] ; Heck, Albert J. R. ^{[2, 3]} ; Maarten Altelaar, A. F. ^{[2, 3]} ; Yatsuda, Ana Patricia ^{[1, 4]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Av Cafe S-N, BR-14040930 Ribeirao Preto, SP - Brazil

^[2] Univ Utrecht, Biomol Mass Spectrometry & Prote, Utrecht Inst Pharmaceut Sci, Padualaan 8, NL-3884 CH Utrecht - Netherlands

^[3] Univ Utrecht, Bijvoet Ctr Biomol Res, Padualaan 8, NL-3884 CH Utrecht - Netherlands

^[4] Univ Sao Paulo, Dept Anal Clin Bromatol & Toxicol, Fac Ciencias Farmaceut Ribeirao Preto, BR-14040930 Ribeirao Preto - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Parasitology; v. 146, n. 1, p. 33-41, JAN 2019.
• Citações Web of Science: 1

Resumo

Apicomplexan parasites have unconventional actins that play a central role in important cellular processes such as apicoplast replication, motility of dense granules, endocytic trafficking and force generation for motility and host cell invasion. In this study, we investigated the actin of the apicomplexan Neospora caninum - a parasite associated with infectious abortion and neonatal mortality in livestock. Neospora caninum actin was detected and identified in two bands by one-dimensional (1D) western blot and in nine spots by the 2D technique. The mass spectrometry data indicated that N. caninum has at least nine different actin isoforms, possibly caused by post-translational modifications. In addition, the C4 pan-actin antibody detected specifically actin in N. caninum cellular extract. Extracellular N. caninum tachyzoites were treated with toxins that act on actin, jasplakinolide and cytochalasin D. Both substances altered the peripheric cytoplasmic localization of actin on tachyzoites. Our findings add complexity to the study of the apicomplexan actin in cellular processes, since the multiple functions of this important protein might be regulated by mechanisms involving post-translational modifications. (AU)

• Processo FAPESP: 12/22772-2 - Identificação e caracterização de proteínas que se ligam à actina (ABPs) no Apicomplexa Neospora caninum
• Beneficiário: Luciana Baroni
• Modalidade de apoio: Bolsas no Brasil - Doutorado