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Genotoxic and epigenotoxic effects in mice exposed to concentrated ambient fine particulate matter (PM2.5) from SAo Paulo city, Brazil

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Autor(es):
Falcao de Oliveira, Antonio Anax [1] ; de Oliveira, Tiago Franco [1, 2] ; Dias, Michelle Francini [1] ; Gennari Medeiros, Marisa Helena [3] ; Di Mascio, Paolo [3] ; Veras, Mariana [4] ; Lemos, Miriam [4] ; Marcourakis, Tania [1] ; Nascimento Saldiva, Paulo Hilario [4, 5] ; Melo Loureiro, Ana Paula [1]
Número total de Autores: 10
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Fac Ciencias Farmaceut, Dept Anal Clin & Toxicol, Ave Prof Lineu Prestes 580, Bloco 13 B, BR-05508000 Sao Paulo - Brazil
[2] Univ Fed Ciencias Saude Porto Alegre, Dept Farmacociencias, Rua Sarmento Leite 245, BR-90050170 Porto Alegre, RS - Brazil
[3] Univ Sao Paulo, Inst Quim, Dept Bioquim, Ave Prof Lineu Prestes 748, BR-05508000 Sao Paulo - Brazil
[4] Univ Sao Paulo, Fac Med, Hosp Clin, Lab Poluicao Atmosfer Expt LIM05, Ave Dr Arnaldo 455, BR-01246903 Sao Paulo - Brazil
[5] Univ Sao Paulo, Inst Estudos Avancados, R Anfiteatro 513, BR-05508060 Sao Paulo - Brazil
Número total de Afiliações: 5
Tipo de documento: Artigo Científico
Fonte: PARTICLE AND FIBRE TOXICOLOGY; v. 15, OCT 19 2018.
Citações Web of Science: 0
Resumo

BackgroundThe Metropolitan Area of SAo Paulo has a unique composition of atmospheric pollutants, and positive correlations between exposure and the risk of diseases and mortality have been observed. Here we assessed the effects of ambient fine particulate matter (PM2.5) on genotoxic and global DNA methylation and hydroxymethylation changes, as well as the activities of antioxidant enzymes, in tissues of AJ mice exposed whole body to ambient air enriched in PM2.5, which was concentrated in a chamber near an avenue of intense traffic in SAo Paulo City, Brazil.ResultsMice exposed to concentrated ambient PM2.5 (1h daily, 3months) were compared to in situ ambient air exposed mice as the study control. The concentrated PM2.5 exposed group presented increased levels of the oxidized nucleoside 8-oxo-7,8-dihydro-2-deoxyguanosine in lung and kidney DNA and increased levels of the etheno adducts 1,N-6-etheno-2-deoxyadenosine and 1,N-2-etheno-2-deoxyguanosine in kidney and liver DNA, respectively. Apart from the genotoxic effects, the exposure to PM2.5 led to decreased levels of the epigenetic mark 5-hydroxymethylcytosine (5-hmC) in lung and liver DNA. Changes in lung, liver, and erythrocyte antioxidant enzyme activities were also observed. Decreased glutathione reductase and increased superoxide dismutase (SOD) activities were observed in the lungs, while the liver presented increased glutathione S-transferase and decreased SOD activities. An increase in SOD activity was also observed in erythrocytes. These changes are consistent with the induction of local and systemic oxidative stress.ConclusionsMice exposed daily to PM2.5 at a concentration that mimics 24-h exposure to the mean concentration found in ambient air presented, after 3months, increased levels of DNA lesions related to the occurrence of oxidative stress in the lungs, liver, and kidney, in parallel to decreased global levels of 5-hmC in lung and liver DNA. Genetic and epigenetic alterations induced by pollutants may affect the genes committed to cell cycle control, apoptosis, and cell differentiation, increasing the chance of cancer development, which merits further investigation. (AU)

Processo FAPESP: 13/07937-8 - Redoxoma
Beneficiário:Ohara Augusto
Linha de fomento: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
Processo FAPESP: 11/09891-0 - Alterações epigenéticas e genotóxicas em DNA de camundongos expostos a material particulado (MP2,5)
Beneficiário:Antonio Anáx Falcão de Oliveira
Linha de fomento: Bolsas no Brasil - Iniciação Científica
Processo FAPESP: 12/08617-4 - Variáveis no controle glicêmico e patogênese da nefropatia diabética: investigação sobre o fenômeno da memória metabólica
Beneficiário:Antonio Anáx Falcão de Oliveira
Linha de fomento: Bolsas no Brasil - Doutorado Direto
Processo FAPESP: 12/21636-8 - Transformação de células epiteliais bronquiais humanas por benzo[a]pireno: processos metabólicos redox e metilação/desmetilação do DNA
Beneficiário:Tiago Franco de Oliveira
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 12/08616-8 - Caracterização de uma nova via de biotransformação do bisfenol A e quantificação de lesões em DNA de células HL-60 e MCF-7
Beneficiário:Ana Paula de Melo Loureiro
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 12/22190-3 - O papel da memória metabólica no desenvolvimento da nefropatia diabética
Beneficiário:Ana Paula de Melo Loureiro
Linha de fomento: Auxílio à Pesquisa - Regular