Effect of photopolymerized glaze application on bacterial adhesion on ocular acrylic resin surfaces submitted to accelerated ageing

Autor(es)

Nagay, B. E. ^[1] ; Goiato, M. C. ^[1] ; da Silva, E. V. F. ^[1] ; Andreotti, A. M. ^[1] ; Bitencourt, S. B. ^[1] ; Duque, C. ^[2] ; dos Santos, P. H. ^[1] ; dos Santos, D. M. ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Aracatuba Dent Sch, Dept Dent Mat & Prosthodont, Jose Bonifacio St, 1153, Vila Mendonca, BR-16015050 Sao Paulo - Brazil

^[2] Sao Paulo State Univ UNESP, Aracatuba Dent Sch, Dept Pediat Dent & Publ Hlth, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Letters in Applied Microbiology; v. 68, n. 2, p. 120-127, FEB 2019.
• Citações Web of Science: 0

Resumo

Conditions of the acrylic resin (AR) surface, such as roughness, can promote a favourable environment for the adhesion of micro-organisms, even on the surface of ocular prostheses. This study evaluated the influence of photopolymerized glaze application on the roughness of ARs and adhesion of Staphylococcus aureus and Staphylococcus epidermidis on ocular AR surfaces submitted to accelerated ageing. Two hundred and eighty-eight samples of white colour (N1) and colourless ARs were distributed in eight groups (n = 9), based on surface treatments (glaze or ARs submitted to only a final polishing), accelerated ageing (before and after) and periods of microbial growth (24- and 48-h). The roughness average (Ra) and total height of roughness profile (Rt) values were greater for the groups with glaze and increased for all groups after ageing. The microbial adhesion among the groups with and without glaze did not present a statistically significant difference. The ageing did not statistically affect the adhesion of Staph. epidermidis, but affected the adhesion of Staph. aureus, which presented an increase after 24 h of growth on only N1 AR with glaze. These results demonstrate that the glaze did not contribute to adhesion of Staph. aureus and Staph. epidermidis, which are responsible for most ocular prosthetic infections. Significance and Impact of the Study Some recent evidence suggested that the surface finish of ocular prostheses influences the accumulation of deposits that can affect the interaction with pathogenic bacteria, increasing the probability of infections. In addition, surface deterioration over time can increase the roughness and, consequently, biofilm formation. Thus, a better understanding of the influence of surface finish on bacterial adhesion becomes extremely important. In this study, we tested a glaze for surface polishing compared to mechanical polishing, before and after ageing. The results suggest that the glaze did not contribute to microbial adhesion and might be useful in preventing possible prosthetic infections. (AU)

• Processo FAPESP: 14/21697-2 - Efeito da aplicação de glaze fotopolimerizável na adesão de Staphylococcus epidermidis e Staphylococcus aureus em superfícies de resinas acrílicas oculares submetidas ao envelhecimento acelerado
• Beneficiário: Bruna Egumi Nagay
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica