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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Antioxidant role on the protection of melanocytes against visible light-induced photodamage

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Autor(es):
Freitas, Juliana Vescovi [1] ; Junqueira, Helena Couto [2] ; Martins, Waleska Kerllen [2, 3] ; Baptista, Mauricio S. [2] ; Gaspar, Lorena Rigo [1]
Número total de Autores: 5
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Ciencias Farmaceut, Ribeirao Preto - Brazil
[2] Univ Sao Paulo, Inst Quim, Dept Bioquim, Sao Paulo - Brazil
[3] Univ Anhanguera Sao Paulo, Sao Paulo - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: Free Radical Biology and Medicine; v. 131, p. 399-407, FEB 1 2019.
Citações Web of Science: 0
Resumo

Visible light can induce the generation of singlet oxygen and can cause oxidative stress, especially in melanocytes due to melanin photosensitization. Currently, there is no organic UV-filter that provide visible light protection. Previous studies showed that some antioxidants, such as apigenin (API), chrysin (CRI) and beta-carotene (BTC) besides neutralizing radical chain reactions can also quench singlet oxygen via physical or chemical quenching and exhibit potential for use in photoprotection. Therefore, the aim of this study is to evaluate the efficacy of API, CRI and BTC on the protection against cell death induced by melanin photosensitization and understand the underlying mechanisms that are involved in the protection. Precise protocols of melanogenesis and quantification of singlet oxygen generation were developed. Viability of B16-F10 cells with melanin basal levels and after melanogenesis induction was evaluated after visible light exposure in the presence and absence of API, CRI and BTC. Results showed that API and BTC protected cells from photoinduced cell death API exhibiting superior photoprotective effect. We noticed that the efficiency of cell protection and the rate of singlet oxygen suppression are not well correlated, at least for the studied series of antioxidants, indicating that the anti-radical capacity should be playing a major role in protecting cells against the damage induced by melanin photosensitization. In terms of sun care strategies, both API and BTC offer protection against visible light-induced damages and may be effective topical antioxidants to be added to sunscreens. (AU)

Processo FAPESP: 13/07937-8 - Redoxoma
Beneficiário:Ohara Augusto
Linha de fomento: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
Processo FAPESP: 16/07642-6 - A ativação/inibição da autofagia pelos triterpenóides e o impacto da interação em membranas: implicações terapêuticas antitumorais
Beneficiário:Waleska Kerllen Martins Gardesani
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 12/50680-5 - Fotossensibilização nas ciências da vida
Beneficiário:Mauricio da Silva Baptista
Linha de fomento: Auxílio à Pesquisa - Temático