Application of Autologous Platelet-Rich Plasma on Tooth Extraction Site Prevents Occurence of Medication-Related Osteonecrosis of the Jaws in Rats

Autor(es):Mostrar menos -

Toro, Luan Felipe ^{[1, 2]} ; de Mello-Neto, Joao Martins ^{[2, 3]} ; Ventura dos Santos, Fernanda Furuse ^[2] ; Ferreira, Leticia Chaves ^[2] ; Statkievicz, Cristian ^[3] ; Angelo Cintra, Luciano Tavares ^[4] ; Mardegan Issa, Joao Paulo ^[5] ; Menegati Dornelles, Rita Cassia ^[2] ; de Almeida, Juliano Milanezi ^[3] ; Hitomi Nagata, Maria Jose ^[3] ; Garcia, Valdir Gouveia ^[3] ; Theodoro, Leticia Helena ^[3] ; Casatti, Claudio Aparecido ^[2] ; Ervolino, Edilson ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Inst Biosci, Rua Prof Dr Antonio Celso Wagner Zanin 250, BR-18618689 Botucatu, SP - Brazil

^[2] Sao Paulo State Univ UNESP, Sch Dent, Dept Basic Sci, Rua Jose Bonifacio 1193, BR-16015050 Aracatuba, SP - Brazil

^[3] Sao Paulo State Univ UNESP, Sch Dent, Dept Surg & Integrated Clin, Rua Jose Bonifacio 1193, BR-16015050 Aracatuba, SP - Brazil

^[4] Sao Paulo State Univ UNESP, Sch Dent, Dept Restorat Dent, Rua Jose Bonifacio 1193, BR-16015050 Aracatuba, SP - Brazil

^[5] Sao Paulo Univ USP, Sch Dent, Dept Morphol Physiol & Basic Pathol, Ave Cafe S-N, BR-14040904 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 9, JAN 10 2019.
• Citações Web of Science: 0

Resumo

This study evaluated the effects of local application of autologous platelet-rich plasma (PRP) on the tooth extraction site of rats presenting the main risk factors for medication-related osteonecrosis of the jaw (MRONJ). For seven weeks, senile rats were submitted to systemic treatment with vehicle (VEH and VEH-PRP) or 100 mu g/Kg of zoledronate (ZOL and ZOL-PRP) every three days. After three weeks, the first lower molar was extracted. VEH-PRP and ZOL-PRP recei`ved PRP at the tooth extraction site. Euthanasia was performed at 28 days postoperatively. Clinical, histopathologica I, histometric and immunohistochemical analyses were carried out in histological sections from the tooth extraction site. ZOL showed lower percentage of newly formed bone tissue (NFBT), higher percentage of nonvital bone tissue (NVBT), as well as higher immunolabeling for TNF alpha and IL-1 beta. In addition, ZOL presented lower immunolabeling for PCNA, VEGF, BMP2/4, OCN and TRAP. VEH and ZOL-PRP showed improvement in the tooth extraction site wound healing and comparable percentage of NFBT, VEGF, BMP2/4 and OCN. Local application of autologous PRP proved a viable preventive therapy, which is safe and effective to restore tissue repair capacity of the tooth extraction site and prevent the occurrence of MRONJ following tooth extraction. (AU)

• Processo FAPESP: 13/26779-4 - Efeito do zoledronato sobre o processo de reparo alveolar: estudo envolvendo os principais fatores de risco para o desenvolvimento de osteonecrose dos maxilares e avaliação da terapia fotodinâmica antimicrobiana (aPDT) como proposta preventiva
• Beneficiário: Edilson Ervolino
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/02199-1 - Avaliação do efeito da Terapia Fotodinâmica Antimicrobiana (aPDT) no processo de reparo alveolar de ratas que apresentam os principais fatores de risco para o desenvolvimento de osteonecrose dos maxilares associada ao uso de bisfosfonatos
• Beneficiário: Cristian Statkievicz
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 14/14542-2 - Aplicação local de plasma rico em plaquetas após exodontia em ratas que apresentam os principais fatores de risco para a osteonecrose dos maxilares associada ao uso de bisfosfonatos: avaliação de uma terapia preventiva
• Beneficiário: Fernanda Furuse Ventura dos Santos
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica