Application of Autologous Platelet-Rich Plasma on Tooth Extraction Site Prevents Occurence of Medication-Related Osteonecrosis of the Jaws in Rats

Author(s):Show less -

Toro, Luan Felipe ^{[1, 2]} ; de Mello-Neto, Joao Martins ^{[2, 3]} ; Ventura dos Santos, Fernanda Furuse ^[2] ; Ferreira, Leticia Chaves ^[2] ; Statkievicz, Cristian ^[3] ; Angelo Cintra, Luciano Tavares ^[4] ; Mardegan Issa, Joao Paulo ^[5] ; Menegati Dornelles, Rita Cassia ^[2] ; de Almeida, Juliano Milanezi ^[3] ; Hitomi Nagata, Maria Jose ^[3] ; Garcia, Valdir Gouveia ^[3] ; Theodoro, Leticia Helena ^[3] ; Casatti, Claudio Aparecido ^[2] ; Ervolino, Edilson ^{[1, 2]}

Affiliation

^[1] Sao Paulo State Univ UNESP, Inst Biosci, Rua Prof Dr Antonio Celso Wagner Zanin 250, BR-18618689 Botucatu, SP - Brazil

^[2] Sao Paulo State Univ UNESP, Sch Dent, Dept Basic Sci, Rua Jose Bonifacio 1193, BR-16015050 Aracatuba, SP - Brazil

^[3] Sao Paulo State Univ UNESP, Sch Dent, Dept Surg & Integrated Clin, Rua Jose Bonifacio 1193, BR-16015050 Aracatuba, SP - Brazil

^[4] Sao Paulo State Univ UNESP, Sch Dent, Dept Restorat Dent, Rua Jose Bonifacio 1193, BR-16015050 Aracatuba, SP - Brazil

^[5] Sao Paulo Univ USP, Sch Dent, Dept Morphol Physiol & Basic Pathol, Ave Cafe S-N, BR-14040904 Ribeirao Preto, SP - Brazil

• Document type: Journal article
• Source: SCIENTIFIC REPORTS; v. 9, JAN 10 2019.
• Web of Science Citations: 0

Abstract

This study evaluated the effects of local application of autologous platelet-rich plasma (PRP) on the tooth extraction site of rats presenting the main risk factors for medication-related osteonecrosis of the jaw (MRONJ). For seven weeks, senile rats were submitted to systemic treatment with vehicle (VEH and VEH-PRP) or 100 mu g/Kg of zoledronate (ZOL and ZOL-PRP) every three days. After three weeks, the first lower molar was extracted. VEH-PRP and ZOL-PRP recei`ved PRP at the tooth extraction site. Euthanasia was performed at 28 days postoperatively. Clinical, histopathologica I, histometric and immunohistochemical analyses were carried out in histological sections from the tooth extraction site. ZOL showed lower percentage of newly formed bone tissue (NFBT), higher percentage of nonvital bone tissue (NVBT), as well as higher immunolabeling for TNF alpha and IL-1 beta. In addition, ZOL presented lower immunolabeling for PCNA, VEGF, BMP2/4, OCN and TRAP. VEH and ZOL-PRP showed improvement in the tooth extraction site wound healing and comparable percentage of NFBT, VEGF, BMP2/4 and OCN. Local application of autologous PRP proved a viable preventive therapy, which is safe and effective to restore tissue repair capacity of the tooth extraction site and prevent the occurrence of MRONJ following tooth extraction. (AU)

• FAPESP's process: 13/26779-4 - Effect of zoledronate during the alveolar healing process in rats: study involving the major risk factors for developing osteonecrosis of the jaws and assessment of antimicrobial photodynamic therapy (aPDT) as preventive therapy
• Grantee: Edilson Ervolino
• Support Opportunities: Regular Research Grants
• FAPESP's process: 14/02199-1 - Evaluation of the effect of Antimicrobial Photodynamic Therapy (aPDT) during the alveolar healing process in rats that presents the major risk factors for developing osteonecrosis of the jaws associated with the use of bisphosphonates
• Grantee: Cristian Statkievicz
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 14/14542-2 - Local application of platelet-rich plasma (PRP) after dental extraction in rats that show the main risk factors for osteonecrosis of the jaws associated with the use of bisphosphonates: evaluation of a preventive therapy
• Grantee: Fernanda Furuse Ventura dos Santos
• Support Opportunities: Scholarships in Brazil - Scientific Initiation