Omega-3 multiple effects increasing glucocorticoid-induced muscle atrophy: autophagic, AMPK and UPS mechanisms

Autor(es)

Fappi, Alan ^[1] ; Neves, Juliana de C. ^[1] ; Kawasaki, Karine A. ^[1] ; Bacelar, Luana ^[1] ; Sanches, Leandro N. ^[1] ; da Silva, Felipe P. ^[1] ; Larina-Neto, Rubens ^[1] ; Chadi, Gerson ^[1] ; Zanoteli, Edmar ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Med FMUSP, Dept Neurol, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PHYSIOLOGICAL REPORTS; v. 7, n. 1 JAN 2019.
• Citações Web of Science: 1

Resumo

Muscle atrophy occurs in many conditions, including use of glucocorticoids. N-3 (omega-3) is widely consumed due its healthy properties; however, concomitant use with glucocorticoids can increase its side effects. We evaluated the influences of N-3 on glucocorticoid atrophy considering IGF-1, Myostatin, MEK/ERK, AMPK pathways besides the ubiquitin-proteasome system (UPS) and autophagic/lysosomal systems. Sixty animals constituted six groups: CT, N-3 (EPA 100 mg/kg/day for 40 days), DEXA 1.25 (DEXA 1.25 mg/kg/day for 10 days), DEXA 1.25 + N3 (EPA for 40 days + DEXA 1.25 mg/kg/day for the last 10 days), DEXA 2.5 (DEXA 2.5 mg/kg/day for 10 days), and DEXA 2.5 + N3 (EPA for 40 days + DEXA 2.5 mg/kg/day for 10 days). Results: N-3 associated with DEXA increases atrophy (fibers 1 and 2A), FOXO3a, P-SMAD2/3, Atrogin-1/MAFbx (mRNA) expression, and autophagic protein markers (LC3II, LC3II/LC3I, LAMP-1 and acid phosphatase). Additionally, N-3 supplementation alone decreased P-FOXO3a, PGC1-alpha, and type 1 muscle fiber area. Conclusion: N-3 supplementation increases muscle atrophy caused by DEXA in an autophagic, AMPK and UPS process. (AU)

• Processo FAPESP: 18/24635-9 - Múltiplos efeitos do Omega-3 aumentando a atrofia induzida por corticoides: mecanismos autofágicos, AMPK e UPS.
• Beneficiário: Edmar Zanoteli
• Modalidade de apoio: Auxílio à Pesquisa - Publicações científicas - Artigo
• Processo FAPESP: 13/23191-6 - Efeitos de glicocorticoides sobre as vias moleculares que regulam o trofismo muscular em ratos e o efeito do ácido graxo ômega-3 (EPA/DHA) na atrofia muscular induzida pela dexametasona
• Beneficiário: Alan Fappi
• Modalidade de apoio: Bolsas no Brasil - Doutorado