| Full text | |
| Author(s): |
Fappi, Alan
[1]
;
Neves, Juliana de C.
[1]
;
Kawasaki, Karine A.
[1]
;
Bacelar, Luana
[1]
;
Sanches, Leandro N.
[1]
;
da Silva, Felipe P.
[1]
;
Larina-Neto, Rubens
[1]
;
Chadi, Gerson
[1]
;
Zanoteli, Edmar
[1]
Total Authors: 9
|
| Affiliation: | [1] Univ Sao Paulo, Fac Med FMUSP, Dept Neurol, Sao Paulo, SP - Brazil
Total Affiliations: 1
|
| Document type: | Journal article |
| Source: | PHYSIOLOGICAL REPORTS; v. 7, n. 1 JAN 2019. |
| Web of Science Citations: | 1 |
| Abstract | |
Muscle atrophy occurs in many conditions, including use of glucocorticoids. N-3 (omega-3) is widely consumed due its healthy properties; however, concomitant use with glucocorticoids can increase its side effects. We evaluated the influences of N-3 on glucocorticoid atrophy considering IGF-1, Myostatin, MEK/ERK, AMPK pathways besides the ubiquitin-proteasome system (UPS) and autophagic/lysosomal systems. Sixty animals constituted six groups: CT, N-3 (EPA 100 mg/kg/day for 40 days), DEXA 1.25 (DEXA 1.25 mg/kg/day for 10 days), DEXA 1.25 + N3 (EPA for 40 days + DEXA 1.25 mg/kg/day for the last 10 days), DEXA 2.5 (DEXA 2.5 mg/kg/day for 10 days), and DEXA 2.5 + N3 (EPA for 40 days + DEXA 2.5 mg/kg/day for 10 days). Results: N-3 associated with DEXA increases atrophy (fibers 1 and 2A), FOXO3a, P-SMAD2/3, Atrogin-1/MAFbx (mRNA) expression, and autophagic protein markers (LC3II, LC3II/LC3I, LAMP-1 and acid phosphatase). Additionally, N-3 supplementation alone decreased P-FOXO3a, PGC1-alpha, and type 1 muscle fiber area. Conclusion: N-3 supplementation increases muscle atrophy caused by DEXA in an autophagic, AMPK and UPS process. (AU) | |
| FAPESP's process: | 13/23191-6 - Glucocorticoid effects on molecular pathways related to the muscular trophysm in rats and the effect of omega-3 fatty acid (EPA/DHA) on dexametasone-induced muscle atrophy |
| Grantee: | Alan Fappi |
| Support Opportunities: | Scholarships in Brazil - Doctorate |