| Texto completo | |
| Autor(es): |
Souza, Deborah S.
[1]
;
Lombardi, Ana Paola G.
[1]
;
Vicente, Carolina M.
[1]
;
Lucas, Thais Fabiana G.
[1]
;
Erustes, Adolfo G.
[1]
;
Pereira, Gustavo J. S.
[1]
;
Porto, Catarina S.
[1]
Número total de Autores: 7
|
| Afiliação do(s) autor(es): | [1] Univ Fed Sao Paulo, Dept Pharmacol, Lab Expt Endocrinol, Escola Paulista Med, Rua Pedro de Toledo 669, Vila Clementino, BR-04039032 Sao Paulo, SP - Brazil
Número total de Afiliações: 1
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Molecular and Cellular Endocrinology; v. 483, p. 11-23, MAR 1 2019. |
| Citações Web of Science: | 1 |
| Resumo | |
The aim of the present study was to investigate the subcellular localization of estrogen receptors ER alpha and ER beta in androgen-independent prostate cancer cell line DU-145, and the possible role of exportin CRM1 on ERs distribution. In addition, we evaluated the ERs contribution to activation of ERK1/2 and AKT. Immunostaining of ER alpha and ER beta was predominantly found in the extranuclear regions of DU-145 cells. CRM1 inhibitor Leptomycin B reduced drastically the presence of ER alpha and ER beta in the extranuclear regions and increased in the nuclei, indicating the possible involvement of CRM1 on ERs nuclear-cytoplasmic shuffling. 17 beta-estradiol (E2), ER alpha-selective agonist PPT and ER beta-selective agonist DPN induced a rapid increase on ERK1/2 phosphorylation. E2-induced ERK1/2 activation was partially inhibited when cells were pretreated with ER alpha- or ER beta-selective antagonists, and blocked by simultaneous pretreatment with both antagonists, suggesting ER alpha/beta heterodimers formation. Furthermore, E2 treatment did not activate AKT pathway. Therefore, we highlighted a possible crosstalk between extranuclear and nuclear ERs and their upstream and downstream signaling molecules as an important mechanism to control ER function as a potential therapeutic target in prostate cancer cells. (AU) | |
| Processo FAPESP: | 14/05292-2 - Receptores estrogênicos e vias de sinalização intracelular envolvidas na regulação da proliferação de células do câncer testicular e do câncer prostático resistente à castração |
| Beneficiário: | Catarina Segreti Porto |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 17/16060-3 - Sinalização intracelular e função dos receptores estrogênicos em células de câncer prostático resistentes à castração e em células de câncer testicular. |
| Beneficiário: | Catarina Segreti Porto |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |