| Full text | |
| Author(s): |
Souza, Deborah S.
[1]
;
Lombardi, Ana Paola G.
[1]
;
Vicente, Carolina M.
[1]
;
Lucas, Thais Fabiana G.
[1]
;
Erustes, Adolfo G.
[1]
;
Pereira, Gustavo J. S.
[1]
;
Porto, Catarina S.
[1]
Total Authors: 7
|
| Affiliation: | [1] Univ Fed Sao Paulo, Dept Pharmacol, Lab Expt Endocrinol, Escola Paulista Med, Rua Pedro de Toledo 669, Vila Clementino, BR-04039032 Sao Paulo, SP - Brazil
Total Affiliations: 1
|
| Document type: | Journal article |
| Source: | Molecular and Cellular Endocrinology; v. 483, p. 11-23, MAR 1 2019. |
| Web of Science Citations: | 1 |
| Abstract | |
The aim of the present study was to investigate the subcellular localization of estrogen receptors ER alpha and ER beta in androgen-independent prostate cancer cell line DU-145, and the possible role of exportin CRM1 on ERs distribution. In addition, we evaluated the ERs contribution to activation of ERK1/2 and AKT. Immunostaining of ER alpha and ER beta was predominantly found in the extranuclear regions of DU-145 cells. CRM1 inhibitor Leptomycin B reduced drastically the presence of ER alpha and ER beta in the extranuclear regions and increased in the nuclei, indicating the possible involvement of CRM1 on ERs nuclear-cytoplasmic shuffling. 17 beta-estradiol (E2), ER alpha-selective agonist PPT and ER beta-selective agonist DPN induced a rapid increase on ERK1/2 phosphorylation. E2-induced ERK1/2 activation was partially inhibited when cells were pretreated with ER alpha- or ER beta-selective antagonists, and blocked by simultaneous pretreatment with both antagonists, suggesting ER alpha/beta heterodimers formation. Furthermore, E2 treatment did not activate AKT pathway. Therefore, we highlighted a possible crosstalk between extranuclear and nuclear ERs and their upstream and downstream signaling molecules as an important mechanism to control ER function as a potential therapeutic target in prostate cancer cells. (AU) | |
| FAPESP's process: | 14/05292-2 - Estrogen receptors and intracellular signaling pathways involved in the regulation of cell proliferation of testicular cancer and castration-resistant prostate cancer |
| Grantee: | Catarina Segreti Porto |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 17/16060-3 - Intracellular signaling and role of the estrogen receptors in androgen-independent prostate cancer and testicular cancer cells. |
| Grantee: | Catarina Segreti Porto |
| Support Opportunities: | Regular Research Grants |