In vitro shoot regeneration from roots and leaf discs of Passiflora cincinnata mast.

Autor(es)

Simone Pacheco Lombardi ^[1] ; Ilene Ribeiro da Silva Passos ^[2] ; Maria Cristina Stolf Nogueira ^[3] ; Beatriz Appezzato-da-Glória ^[4]

Afiliação do(s) autor(es)

^[1] Universidade de São Paulo. Escola Superior de Agricultura Luiz de Queiroz. Departamento de Ciências Biológicas - Brasil

^[2] Instituto Agronômico de Campinas. Centro de Pesquisa e Desenvolvimento de Recursos Genéticos Vegetais

^[3] USP. ESALQ. Departamento de Ciências Exatas - Brasil

^[4] Universidade de São Paulo. Escola Superior de Agricultura Luiz de Queiroz. Departamento de Ciências Biológicas - Brasil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Archives of Biology and Technology; v. 50, n. 2, p. 239-247, 2007-03-00.

Resumo

Different concentrations of 6-benzyladenine (6-BA) were used in vitro to induce buds in three types of explants: leaf discs, root segments and the seedling itself obtained from in vitro seed germination of Passiflora cincinnata Mast. The 0.5 mg.L -1 6-BA concentration was most suitable for all the three explants, however, bud formation time and means (direct/indirect) were different for each type of explant. In seedlings used as explants, it had shoot regeneration from the primary root. Histological events leading to bud formation via meristemoids were described. The origin was indirect in leaf discs, with callus formation in subepidermal cells of the chlorophyll parenchyma layers. In root segments and in seedling roots, the buds were formed directly in the pericycle (in roots with some secondary structure) and in the vascular cambium (at the initial phase of secondary structure). Also, indirect buds originated from meristemoids which were formed around the callus. (AU)

• Processo FAPESP: 00/05780-4 - Otimização de protocolos de técnicas de cultura de tecidos de Passiflora spp., visando sua utilização no melhoramento genético do maracujá
• Beneficiário: Ilene Ribeiro da Silva Passos
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 02/05940-7 - Estudos anatômicos e fisiológicos da organogênese in vitro em Passiflora cincinatta Mast.
• Beneficiário: Simone Pacheco Lombardi
• Modalidade de apoio: Bolsas no Brasil - Mestrado