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Analysis of Defense-Related Gene Expression in Citrus Hybrids Infected by Xylella fastidiosa

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Autor(es):
Mauricio, F. N. [1, 2] ; Soratto, T. A. T. [1, 2] ; Diogo, J. A. [1] ; Boscariol-Camargo, R. L. [1] ; De Souza, A. A. [1] ; Coletta-Filho, H. D. [1] ; Silva, J. A. A. [3] ; Medeiros, A. H. [2] ; Machado, M. A. [1] ; Cristofani-Yaly, M. [1]
Número total de Autores: 10
Afiliação do(s) autor(es):
[1] Ctr Citricultura Sylvio Moreira IAC, CP 04, BR-13490970 Cordeiropolis, SP - Brazil
[2] Univ Fed Sao Carlos, Campus Araras, Rodovia Anhanguera, Km 174, Araras, SP - Brazil
[3] Polo Reg Alta Mogiana, CP 35, Colina, SP - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: PHYTOPATHOLOGY; v. 109, n. 2, p. 301-306, FEB 2019.
Citações Web of Science: 1
Resumo

Resistance to Xylella fastidiosa was evaluated in 264 hybrids of crosses between Murcott tangor (Citrus reticulata x Citrus sinensis) and Pera sweet orange (C. sinensis) under field conditions. Uninfected hybrids were grafted with buds collected from Pera sweet orange plants infected with X. fastidiosa, forming a plant with two scions (i.e., hybrid branches and Pera sweet orange branches). From these plants, we chose 10 genotypes with three biological replicates. We evaluated gene expression, bacterial multiplication, and citrus variegated chlorosis (CVC) symptom development in both scions. X. fastidiosa was not detected in most hybrid scions and none showed disease symptoms. In contrast, all Pera sweet orange scions were infected with X. fastidiosa and expressed symptoms of CVC. We quantified the expression of 12 defense-related genes by qPCR comparing resistant to susceptible scions. We suggest that some of these genes are involved in resistance of the hybrids to X. fastidiosa, since their expression was significantly higher in the resistant hybrid scions than in tolerant hybrids and scions originated from CVC symptomatic Pera sweet orange buds. However, we note that these data should be interpreted carefully, as the plant genotypes tested are related but necessarily distinct (hybrids of C. reticulata and C. sinensis, in relation to a C. sinensis control). A principal component analysis revealed a relationship between the expression of these genes and hybrid scions, and between scions that originated from infected buds and the presence of the bacteria and plant symptoms. Multiyear field trials are necessary to develop plant resistance to X. fastidiosa. While the experimental design used here had limitations, it allowed us to identify a set of genes potentially involved in Citrus sp. resistance to this pathogen. Future work on the role of these genes in plant defenses to X. fastidiosa infection is necessary to confirm their importance in the displayed resistance phenotype. (AU)

Processo FAPESP: 11/18605-0 - Obtenção e avaliação de novas variedades copas e porta-enxertos para citricultura de mesa
Beneficiário:Mariângela Cristofani-Yaly
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 14/50880-0 - INCT 2014: de genômica comparativa e funcional e melhoramento assistido de citros
Beneficiário:Marcos Antonio Machado
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 07/08435-5 - Resistência às doenças em citros: mapeamento genético e genômica funcional de novos híbridos
Beneficiário:Mariângela Cristofani-Yaly
Linha de fomento: Auxílio à Pesquisa - Regular