MALDI-MSI: a fast and reliable method for direct melatonin quantification in biological fluids

Autor(es)

Ferreira, Monica Siqueira ^[1] ; de Oliveira, Diogo Noin ^[1] ; Mesquita, Caroline Costa ^[2] ; de Lima Barbosa, Ana Paula ^[2] ; Anhe, Gabriel Forato ^[2] ; Catharino, Rodrigo Ramos ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Sch Pharmaceut Sci, INNOVARE Biomarkers Lab, Campinas, SP - Brazil

^[2] Univ Estadual Campinas, Coll Med Sci, Diabet Mellitus Pharmacol Lab, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF ANALYTICAL SCIENCE AND TECHNOLOGY; v. 7, 2016.
• Citações Web of Science: 0

Resumo

Background: This work reports, for the first time, a validated methodology for qualitative and quantitative melatonin analyses directly from a complex matrix, namely rat milk, using matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI), in a useful strategy for fast assessment of this molecular species. Methods: This novel approach uses the imaging feature of a MALDI instrument to create bidimensional images of a selected area. Results for sample concentration are expressed as a function of pixel-by-pixel intensity in grayscale, which are quantified according to pixel intensity through ImageJ open source software. For the extent of this work, two conditions were monitored: light-and dark-obtained milk. Results: Quantification was performed using the selected reaction monitoring (SRM) transitions m/z 233. 174 for melatonin. Limit of detection and limit of quantification have presented lower values (picomolar) when compared to other melatonin quantification strategies, indicating that this method presents high sensitivity. Furthermore, this method has a total analysis time of less than 1 min. Conclusions: Melatonin concentrations are significantly higher in samples obtained from dark conditions, consistent with previous results, and thus proving that MALDI-MSI is a suitable, simple, and highly sensitive methodology for direct melatonin analysis from complex biological samples. (AU)

• Processo FAPESP: 14/00084-2 - Identificação de adulterações e determinação simultânea de nutrientes e contaminantes em alimentos por análise direta em espectrometria de massas e imagem
• Beneficiário: Diogo Noin de Oliveira
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 11/50400-0 - Metabolismo energético, estado redox e funcionalidade mitocondrial na morte celular e em desordens cardiometabólicas e neurodegenerativas
• Beneficiário: Aníbal Eugênio Vercesi
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 15/06809-1 - Determinação de marcadores químicos em diferentes produtos bovinos por Espectrometria de Massas
• Beneficiário: Rodrigo Ramos Catharino
• Modalidade de apoio: Auxílio à Pesquisa - Regular