| Texto completo | |
| Autor(es): |
Tognon-Miguel, Vania
[1]
;
Nascimento-Elias, Adriana H.
[1, 2]
;
Schiavoni, Maria C. L.
[1]
;
Barreiro, Amilton A.
[1]
Número total de Autores: 4
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Med Sch Ribeirao Preto, Dept Neurosci & Behav Sci, Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Med Sch Ribeirao Preto, Dept Biomech Med & Rehabil Locomotor Syst, Ribeirao Preto, SP - Brazil
Número total de Afiliações: 2
|
| Tipo de documento: | Artigo Científico |
| Fonte: | PLASTIC AND RECONSTRUCTIVE SURGERY-GLOBAL OPEN; v. 7, n. 1 JAN 2019. |
| Citações Web of Science: | 0 |
| Resumo | |
Background: Histomorphometric studies of unmyelinated fibers of the rat fibular nerves are uncommon, and side-to-end neurorrhaphy studies using the fibular nerve investigate primarily motor fibers. We investigated side-to-end tubulization (SET) technique, in which occurs collateral sprouting from the intact donor nerve fibers to the distal stump of receptor nerve, with muscle reinnervation and functional rehabilitation, to assess whether there is a successful growth of unmyelinated fibers in this model. Methods: Adult Wistar rats fibular nerves were sectioned to create a 5-mm gap. A 6-mm silicone tube was attached between a side of the intact tibial nerve and the sectioned fibular nerve distal stump (SET group), with the left fibular nerve as normal (sham group). Seventy days postsurgery, unmyelinated fibers from the distal segment of the fibular nerve were quantified using light and transmission electron microscopy and their diameters were measured. Results: The number of unmyelinated fibers was similar between sham (1,882 +/- 270.9) and SET (2,012 +/- 1,060.8), but axons density was significantly greater in the SET (18,733.3 +/- 5,668.6) than sham (13,935.0 +/- 1,875.8). Additionally, the axonal diameters differed significantly between groups with mean measures in sham (0.968 +/- 0.10) > SET (0.648 +/- 0.08). Conclusions: Unmyelinated fiber growth occurred even with a 5-mm distance between the donor and receptor nerves, reaching similar axonal number to the normal nerve, demonstrating that the SET is a reliable technique that can promote a remarkable plasticity of unmyelinated axons. (AU) | |
| Processo FAPESP: | 12/11012-7 - Identificação de veículos farmacêuticos para substâncias e fatores indutores de regeneração do nervo periférico com o uso de tubulização e novo método de estudo da regeneração através da neurorrafia término-lateral |
| Beneficiário: | Amilton Antunes Barreira |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |