MicroRNA-1254 contributes to the controlling of pro-fibrogenic environment in LX-2 cells by modulating SMAD3 and wound repair: new insights in hepatic fibrosis

Autor(es)

Goncalves, Leticia Rocha ^[1] ; da Silva, Brenda de Oliveira ^[2] ; Scarinci, Leticia Destefani ^[1] ; Ramos, Leticia Ferreira ^[1] ; Moraes, Karen C. M. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, Inst Biociencias, Dept Biol, Lab Biol Mol, Campus Rio Claro, BR-13506900 Rio Claro, SP - Brazil

^[2] Univ Fed Ouro Preto, Nucl Pesquisa Biol, Ouro Preto, MG - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Cell Biology International; v. 43, n. 3, p. 333-343, MAR 2019.
• Citações Web of Science: 1

Resumo

Hepatic fibrosis and its end-stage cirrhosis have increased worldwide, and, despite all the efforts, no successful therapy is available. More recently, the heptapeptide angiotensin-(1-7) {[}ang-(1-7)] was reported to be able to modulate liver fibrosis and even steatosis; however, the molecular bases of these effects are not clear. In this study, we investigated the overexpression of the microRNA-1254 in the human hepatic stellate cell line LX-2, based on the effect of the heptapeptide in such cells, previously, demonstrated by our research group. In addition, this miRNA was chosen based on the identification of putative binding site of this small molecule in the mRNA sequences of different molecular connectors of the AKT/ PI3K pathway, which is modulated by the heptapeptide and connects to the control of several cellular mechanisms, including proliferation, survival, migration, and even liver fibrogenesis. The results revealed an innovative function of the miR-1254 in controlling SMAD3 and pro-fibrosing elements as well as the wound healing response in LX-2, attenuating the scaring repair of the injured tissue. The combined findings provide useful information for future studies on the controlling of hepatic fibrogenesis. (AU)

• Processo FAPESP: 13/21186-5 - Redes de interações moleculares reguladas por pequenos RNAs não decodificadores no mecanismo fibrosante hepático e suas modulações pelo peptídeo vasoativo angiotensina-(1-7)
• Beneficiário: Karen Cristiane Martinez de Moraes
• Modalidade de apoio: Auxílio à Pesquisa - Regular